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CD3 anticorps (Cytoplasmic Domain)

CD3 Reactivité: Humain, Souris, Poulet, Porc, Chien, Cheval FACS, IHC (fro), IF, WB, IHC (p) Hôte: Rat Monoclonal CD3-12 unconjugated
N° du produit ABIN119451
  • Antigène Voir toutes CD3 Anticorps
    CD3
    Épitope
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivité
    • 222
    • 96
    • 31
    • 14
    • 14
    • 10
    • 8
    • 7
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    Humain, Souris, Poulet, Porc, Chien, Cheval
    Hôte
    • 258
    • 51
    • 14
    • 13
    • 10
    Rat
    Clonalité
    • 334
    • 8
    Monoclonal
    Conjugué
    • 97
    • 36
    • 32
    • 26
    • 24
    • 13
    • 9
    • 9
    • 9
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CD3 est non-conjugé
    Application
    • 294
    • 93
    • 58
    • 52
    • 45
    • 44
    • 38
    • 24
    • 16
    • 10
    • 9
    • 9
    • 8
    • 7
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Séquence
    Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys
    Specificité
    This antibody recognises a highly conserved epitope of the CD3 Molecule expressed by T lymphocytes. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross reactivity for the CD3 marker. (Jones et al. 1993, Kothlow et al. 2005).
    Réactivité croisée (Details)
    Species reactivity (expected):Mammals, Birds.
    Species reactivity (tested):Human, Mouse, Canine, Chicken, Equine, Porcine and Rhesus Monkey, Alpaca, Amazon Parrot, Duck, Harbour Porpoise, Koala, Raccoon, Sea Lion, Spotted Hyena.
    Purification
    Affinity Chromatography on Protein G
    Immunogène
    Synthetic peptide sequence derived from Cytoplasmic epitope of CD3
    Clone
    CD3-12
    Isotype
    IgG1
    Top Product
    Discover our top product CD3 Anticorps primaire
  • Indications d'application
    Flow cytometry (1/50-1/100, requires membrane permeabilisation). Immunohistochemistry on frozen sections and paraffin embedded tissues (1/1000). This product requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. 1 mM EDTA pH 8.0 is recommended for this purpose. Recommended positive control tissue: Tonsil. Western blotting.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, 0.09 % Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store the reconstituted antibody undiluted at 2-8 °C.
  • Hebel, Eisinger, Neumeier, Rein-Fischboeck, Pohl, Meier, Boettcher, Froehner, Adams, Liebisch, Krautbauer, Buechler: "Lipid abnormalities in alpha/beta2-syntrophin null mice are independent from ABCA1." dans: Biochimica et biophysica acta, Vol. 1851, Issue 5, pp. 527-36, (2015) (PubMed).

    Hong, Puaux, Huang, Loumagne, Tow, Mackay, Kato, Prévost-Blondel, Avril, Nardin, Abastado: "Chemotherapy induces intratumoral expression of chemokines in cutaneous melanoma, favoring T-cell infiltration and tumor control." dans: Cancer research, Vol. 71, Issue 22, pp. 6997-7009, (2011) (PubMed).

    Shrestha, Hashiguchi, Ito, Miura, Takenouchi, Oyama, Kawahara, Tancharoen, Ki-I, Arimura, Yoshinaga, Noma, Shrestha, Nitanda, Kitajima, Arimura, Sato, Sakamoto, Maruyama: "B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4819-26, (2010) (PubMed).

  • Antigène
    CD3
    Autre désignation
    CD3 (CD3 Produits)
    Synonymes
    anticorps CD3, anticorps AI504783, anticorps CD3epsilon, anticorps T3e, anticorps cd3-gamma, anticorps cd3g, anticorps cd3g-a, anticorps cd3g/d, anticorps cd3gd, anticorps t3g, anticorps CD3e molecule, anticorps CD3d molecule, anticorps CD3 antigen, epsilon polypeptide, anticorps CD3g molecule S homeolog, anticorps CD3E, anticorps CD3D, anticorps Cd3e, anticorps cd3g.S
    Sujet
    T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
    ID gène
    916
    NCBI Accession
    NP_000724
    UniProt
    P07766
    Pathways
    TCR Signaling, Ubiquitin Proteasome Pathway
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