This antibody recognises CD107b, also known as MAC-3 and LAMP-2.
Réactivité croisée (Details)
Species reactivity (tested):Mouse
Purification
Affinity chromatography on Protein G
Immunogène
Glycoproteins purified from mouse peritoneal macrophage membranes. Spleen cells from immunised Lewis rats were fused with cells of the mouse P3-NSI/1-Ag4-1 myeloma cell line.
LAMP2
Reactivité: Humain
WB, IHC, ELISA, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL,Results may be enhanced using permeabilisation. Immunoprecipitation. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Antigène
LAMP2
(Lysosomal-Associated Membrane Protein 2 (LAMP2))
CD107b is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between 92-120kD. Studies suggest that CD107b may be involved in leucocyte adhesion.Synonyms: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2