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PAG1 anticorps (AA 97-432)

PAG1 Reactivité: Humain WB, IHC (p), FACS Hôte: Souris Monoclonal MEM-255 unconjugated
N° du produit ABIN120652
  • Antigène Voir toutes PAG1 Anticorps
    PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
    Épitope
    • 16
    • 10
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 97-432
    Reactivité
    • 28
    • 27
    • 24
    • 12
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 43
    • 4
    Souris
    Clonalité
    • 43
    • 4
    Monoclonal
    Conjugué
    • 19
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp PAG1 est non-conjugé
    Application
    • 34
    • 16
    • 13
    • 13
    • 12
    • 8
    • 6
    • 6
    • 5
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
    Specificité
    The antibody recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).
    Purification
    Protein-A affinity chromatography
    Pureté
    > 95 % pure (by SDS-PAGE)
    Immunogène
    Recombinant intracellular fragment (aa 97-432) of human Cbp/PAG
    Clone
    MEM-255
    Isotype
    IgG2a
    Top Product
    Discover our top product PAG1 Anticorps primaire
  • Indications d'application
    Flow Cytometry: 2 mg/mL (intracellular staining, permeabilization required). Western Blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS- PAGE gels anomalously as an 80 kDa molecule. Immunohistochemistry (paraffin sections).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf Life: one year from despatch.
    Date de péremption
    12 months
  • Antigène
    PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
    Autre désignation
    PAG1 / Csk-Binding Protein (PAG1 Produits)
    Synonymes
    anticorps Cbp, anticorps F730007C19Rik, anticorps Pag, anticorps CBP, anticorps PAG, anticorps MGC80192, anticorps phosphoprotein associated with glycosphingolipid microdomains 1, anticorps phosphoprotein membrane anchor with glycosphingolipid microdomains 1, anticorps phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog, anticorps Pag1, anticorps PAG1, anticorps pag1.L, anticorps pag1
    Sujet
    PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.Synonyms: CBP, PAG, Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp
    ID gène
    55824
    UniProt
    Q9NWQ8
    Pathways
    Signalisation p53, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
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