ASAP1 anticorps (AA 775-800)

N° du produit ABIN129670

N° du produit (Fournisseur): 600-401-911

Cet anticorps Lapin Polyclonal détecte spécifiquement ASAP1 dans WB, ELISA, IF et FM. Il présente une réactivité avec des échantillons de Souris.Il a été cité dans 1 publication.

Aperçu rapide pour ASAP1 anticorps (AA 775-800) (ABIN129670)

Antigène: ASAP1 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1 (ASAP1))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ASAP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-ASAP1 anticorps

Épitope: AA 775-800

N° du produit (Fournisseur): 600-401-911

Fournisseur: Rockland

Fonction: Asap1 Antibody

Réactivité croisée (Details): This affinity-purified antibody is directed against mouse ASAP1 protein.

Attributs du produit: Synonyms: rabbit anti-ASAP1 Antibody, ASAP-1, ASAP 1, Development and differentiation enhancing factor 1 antibody, 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein antibody, ADP ribosylation factor directed GTPase activating protein 1 antibody, AMAP 1 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of mouse ASAP1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA, IF microscopy and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to ASAP1 protein by western blotting in the appropriate cell lysate or extract.  This antibody recognizes both phosphorylated and non-phosphorylated ASAP1 at amino acid Y782.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.95 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-ASAP1 (ABIN129670)

Sato, Zhao, Imai, Simister, Feller, Trackman, Kirsch, Sonenshein: "Inhibition of CIN85-mediated invasion by a novel SH3 domain binding motif in the lysyl oxidase propeptide." dans: PLoS ONE, Vol. 8, Issue 10, pp. e77288, (2013) (PubMed).

Détails sur ASAP1

Antigène: ASAP1 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 1 (ASAP1))

Autre désignation: Asap1

Sujet: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. ASAP1 (also known as AMAP1, 130- kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP, ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, Differentiation-enhancing factor 1, DEF-1) is an Arf-directed GTPase activating protein that is a substrate for the kinases Src and FAK and has been implicated in the regulation of membrane traffic, focal adhesions and invadopodia/podosomes.  Phosphorylation of ASAP1 at tyrosine 782 has been found to affect enzymatic and some biological activities, including the function of invadopodia. ASAP1 is expressed in many tissues but is most abundant in the testis, brain, lung and spleen. A heightened expression was seen in the adipose tissue from obese (ob) and diabetic (db) animals. Multiple transcript variants have been reported for this protein.

ID gène: 13196, 65301464

UniProt: Q9QWY8