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Phosphotyrosine anticorps (HRP)

WB, IHC, ELISA Hôte: Souris Monoclonal PY20 HRP
N° du produit ABIN135060
  • Antigène Tous les produits Phosphotyrosine
    Phosphotyrosine
    Reactivité
    Veuillez nous consulter SVP
    Hôte
    • 96
    • 28
    • 1
    Souris
    Clonalité
    • 108
    • 17
    Monoclonal
    Conjugué
    • 45
    • 16
    • 11
    • 8
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Phosphotyrosine est conjugé à/à la HRP
    Application
    • 88
    • 65
    • 41
    • 41
    • 39
    • 28
    • 25
    • 17
    • 13
    • 9
    • 9
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Attributs du produit
    The antibody detects: Phosphorylated tyrosine and phenylphosphate
    Purification
    Purified
    Clone
    PY20
    Isotype
    IgG2b
  • Indications d'application
    Working Dilution:
    ELISA: 1:1,000-1:4,000
    Representative data are included in this product insert.

    Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
    Volume d'échantillon
    1 mL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Product is supplied as 1.0 mL of stock solution in 50 % glycerol / 50 % PBS, pH 7.4.
    Conseil sur la manipulation
    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Stock
    4 °C
  • Antigène
    Phosphotyrosine
    Abstract
    Phosphotyrosine Produits
    Classe de substances
    Amino Acid
    Sujet
    Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.
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