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RRNA anticorps

Détails pour le produit réf. ABIN152086, Fournisseur: Connectez-vous pour afficher
Antigène
Reactivité
Toutes les espèces, Humain, Souris
19
18
18
5
Hôte
Souris
24
Clonalité (Clone)
Monoclonal ()
Conjugué
Inconjugué
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
24
23
23
13
13
Fournisseur
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Immunogène The whole 5.8S ribosomal RNA.
Clone Y10b
Isotype IgG3 kappa
Specificité This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
Purification Protein A purified
Indications d'application Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:2500, Immunoprecipitation 10 μL/lysate, Immunohistochemistry-Paraffin, Flow (Intracellular)
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocole Immunocytochemistry Protocol Specific for rRNA Antibody (Y10b) Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 1M Tris, 1.5M NaCl, 100 mM Glycine
Buffer contains: 0.05 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not freeze.
Stock 4 °C
Stockage commentaire Store at 4°C. Do not freeze.
Produit citée dans: Mastroeni, Grover, Delvaux, Whiteside, Coleman, Rogers: "Epigenetic changes in Alzheimer's disease: decrements in DNA methylation." dans: Neurobiology of aging, Vol. 31, Issue 12, pp. 2025-37, 2010 (PubMed). (Échantillon (espèces): Human).

Jablonka, Wiese, Sendtner: "Axonal defects in mouse models of motoneuron disease." dans: Journal of neurobiology, Vol. 58, Issue 2, pp. 272-86, 2004 (PubMed).

Sotelo-Silveira, Calliari, Cárdenas, Koenig, Sotelo: "Myosin Va and kinesin II motor proteins are concentrated in ribosomal domains (periaxoplasmic ribosomal plaques) of myelinated axons." dans: Journal of neurobiology, Vol. 60, Issue 2, pp. 187-96, 2004 (PubMed). (Échantillon (espèces): Fish). Détails: Immunocytochemistry,Immunofluorescence

Anderson, Merhege, Morin, Bolognani, Perrone-Bizzozero: "Increased expression and localization of the RNA-binding protein HuD and GAP-43 mRNA to cytoplasmic granules in DRG neurons during nerve regeneration." dans: Experimental neurology, Vol. 183, Issue 1, pp. 100-8, 2003 (PubMed).

Zheng, Kelly, Chang, Ryazantsev, Rajasekaran, Martin, Twiss: "A functional role for intra-axonal protein synthesis during axonal regeneration from adult sensory neurons." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 23, pp. 9291-303, 2001 (PubMed). (Échantillon (espèces): Rat (Rattus)). Détails: Immunocytochemistry,Immunoprecipitation,Immunofluorescence

Stone, Rubel: "Temporal, spatial, and morphologic features of hair cell regeneration in the avian basilar papilla." dans: The Journal of comparative neurology, Vol. 417, Issue 1, pp. 1-16, 2000 (PubMed). (Échantillon (espèces): Chicken). Détails: Immunocytochemistry,Immunofluorescence

Gallouzi, Brennan, Stenberg, Swanson, Eversole, Maizels, Steitz: "HuR binding to cytoplasmic mRNA is perturbed by heat shock." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3073-8, 2000 (PubMed).

Reference: Background Bleher, Martin: "Ribosomes in the squid giant axon." dans: Neuroscience, Vol. 107, Issue 3, pp. 527-34, 2001 (PubMed).

Fan, Steitz: "Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs." dans: The EMBO journal, Vol. 17, Issue 12, pp. 3448-60, 1998 (PubMed).