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Caspase 9 anticorps (Caspase 9, Apoptosis-Related Cysteine Peptidase)

Détail du produit anti-CASP9 anticorps No. ABIN153015, Fournisseur: Connectez-vous pour afficher
Antigène
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Alternatives
anti-Humain Caspase 9 anticorps pour Western Blotting
Reactivité
Humain
413
117
114
19
13
5
4
4
3
2
2
2
1
1
1
1
Hôte
Souris
413
47
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp Caspase 9 est non-conjugé
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
384
162
128
78
69
68
45
34
13
13
8
6
2
2
2
Fournisseur
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Immunogène Recombinant human Caspase 9 pro-domain protein. [UniProt# P55211]
Clone LAP6 96-2-22
Isotype IgG1 kappa
Purification Ascites
Autre désignation Caspase-9 (CASP9 Antibody Extrait)
Sujet Gene Symbol: CASP9
ID gène 842
UniProt P55211
Domaine de recherche Apoptosis/Necrosis, Cancer
Pathways Signalisation MAPK, Signalisation RTK, Apoptose, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Indications d'application Western Blot 1:500-1:1000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:50-1:250, Immunohistochemistry-Paraffin 1:50-1:250
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocole Western protocol specific for Caspase 9 Antibody Procedure Guide for NB 500-209 Monoclonal anti-Caspase 9

Western Protocol

1) Run a 10-*15 % SDS polyacrylamide gel, loading ~20 µg of cell extract per lane.
2) Transfer the proteins to a membrane.
3) Block the membrane in PT-T20 [20 mM Tris-HCl, pH 7.4 / 150 mM NaCl/0.5 % Tween 20] + 5 % NFDM [non-fat dry milk], for 3 hour at room temperature (RT), shaking gently.
4) Rinse the membrane twice with PT-T20.
5) Incubate the membrane in anti-Caspase 9 [NB 500-209], diluted 1:500-1:1,000 in PT-T20 + 5 % NFDM, for 60 minutes at RT.
6) Wash the membrane for 15 minutes, 3 times, in PT-T20 at RT.
7) Incubate the membrane in anti-mouse conjugated to HRP (secondary antibody), diluted in PT-T20 + 5 % NFDM for 60 minutes at RT.
8) Wash the membrane for 15 minutes, 3 times, in PT-T20 at RT.
9) Develop membrane in chemiluminescent reagents, as instructed by kit-vendor.

*Positive control(s): Human kidney 293 cells

To Activate the Cell Extracts:
Bring the extract to a final concentration of 5 mM dATP.
Incubate the extracts at 37?C for 15-30 minutes.Immunocytochemistry/Immunofluorescence Protocol for Caspase 9 Antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 30.7 mg/mL
Buffer Buffer contains: 0.1 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock -20 °C,-80 °C
Stockage commentaire Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Caspase 9 anticorps (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). La...
Western Blotting (WB) image for anti-Caspase 9 anticorps (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [ABIN153015] - Western blot analysis ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 9 anticorps (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) Immunohistochemistry-Paraffin: Caspase 9 Antibody (LAP6 96-2-22) [ABIN153015] - IHC a...
Immunofluorescence (IF) image for anti-Caspase 9 anticorps (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) Immunocytochemistry/Immunofluorescence: Caspase 9 Antibody (LAP6 96-2-22) [ABIN153015...
Produit citée dans: Hsuan, Chyau, Hung, Chen, Chou: "The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer." dans: European journal of nutrition, Vol. 55, Issue 2, pp. 491-503, 2016 (PubMed). (Échantillon (espèces): Human). Détails: Western Blotting

Kim, Lee, Park, Chung, Im, Noh, Han, Moon, Jung, Ryu: "Expression of SIRT1 and apoptosis-related proteins is predictive for lymph node metastasis and disease-free survival in luminal A breast cancer." dans: Virchows Archiv : an international journal of pathology, Vol. 467, Issue 5, pp. 563-70, 2015 (PubMed). (Échantillon (espèces): Human). Détails: Immunohistochemistry (Paraffin-embedded Sections)

Martin, Fearnhead: "Apocytochrome c blocks caspase-9 activation and Bax-induced apoptosis." dans: The Journal of biological chemistry, Vol. 277, Issue 52, pp. 50834-41, 2002 (PubMed). (Échantillon (espèces): Human). Détails: Western Blotting

Reference: Background Kirsch, Doseff, Chau, Lim, de Souza-Pinto, Hansford, Kastan, Lazebnik, Hardwick: "Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c." dans: The Journal of biological chemistry, Vol. 274, Issue 30, pp. 21155-61, 1999 (PubMed).

Fearnhead, Rodriguez, Govek, Guo, Kobayashi, Hannon, Lazebnik: "Oncogene-dependent apoptosis is mediated by caspase-9." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 23, pp. 13664-9, 1998 (PubMed).