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Prostate Specific Antigen anticorps

PSA Reactivité: Humain ELISA, WB, FACS, DB Hôte: Souris Monoclonal A5D5-1 unconjugated
N° du produit ABIN1543584
  • Antigène Voir toutes Prostate Specific Antigen (PSA) Anticorps
    Prostate Specific Antigen (PSA)
    Reactivité
    • 189
    • 15
    • 10
    • 5
    • 4
    • 1
    Humain
    Hôte
    • 117
    • 65
    • 4
    • 4
    • 2
    Souris
    Clonalité
    • 131
    • 61
    Monoclonal
    Conjugué
    • 101
    • 17
    • 13
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Prostate Specific Antigen est non-conjugé
    Application
    • 90
    • 76
    • 37
    • 37
    • 26
    • 25
    • 24
    • 14
    • 13
    • 9
    • 9
    • 9
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Flow Cytometry (FACS), Dot Blot (DB)
    Purification
    Protein A affinity column
    Immunogène
    Native PSA Antigen
    Clone
    A5D5-1
    Isotype
    IgG1
  • Commentaires

    Other Applications have not been tested.

    Restrictions
    For Research Use only
  • Validation #029617 (Flow Cytometry)
    'Independent Validation' signe
    by
    Flow Cytometry & Cell Separation Facility, Purdue University
    No.
    #029617
    Date
    22.02.2014
    Antigène
    Numéro du lot
    FAPS-A5D5-0003
    Application validée
    Flow Cytometry
    Contrôle positif
    LNCaP cells
    Contrôle négative
    - DU145 cells - Jurkat cells
    Conclusion
    Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antibody: Prostate Specific Antigen (PSA)
    • Catalog number: ABIN1543584
    • Lot number: FAPS-A5D5-0003
    Anticorps secondaire
    • Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647
    Full Protocol
    • Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
    • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
    • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
    • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
    • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
    • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
    • 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
    • Cells were washed twice with PBS/BSA/Tween 20
    • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
    • Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
    • Cells were washed twice with PBS/BSA/Tween 20
    • Cells were resuspended in 0.3 mL PBS/BSA
    • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
    Notes
    None
  • Format
    Liquid
    Concentration
    1.8 mg/mL
    Buffer
    PBS, pH 7.4, 0.09% sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
  • Antigène
    Prostate Specific Antigen (PSA)
    Autre désignation
    PSA (PSA Produits)
    Synonymes
    anticorps APS, anticorps KLK2A1, anticorps PSA, anticorps hK3, anticorps KLK3, anticorps Klk1c10l2, anticorps RSGK-50, anticorps RSKG-50, anticorps rGK-3, anticorps Kal-3, anticorps Kal3, anticorps Klk3, anticorps AAP-S, anticorps MP100, anticorps Psa, anticorps R74825, anticorps goku, anticorps kallikrein related peptidase 3, anticorps kallikrein 3, anticorps kallikrein 1-related peptidase C3, anticorps kallikrein B, plasma 1, anticorps aminopeptidase puromycin sensitive, anticorps KLK3, anticorps Klk1c3, anticorps Klkb1, anticorps Npepps
    Pathways
    Système du Complément
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