Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Podoplanin anticorps (Biotin)

PDPN Reactivité: Humain FACS Hôte: Souris Monoclonal 18H5 Biotin
N° du produit ABIN155160
  • Antigène Voir toutes Podoplanin (PDPN) Anticorps
    Podoplanin (PDPN)
    Reactivité
    • 89
    • 28
    • 4
    • 3
    • 1
    Humain
    Hôte
    • 56
    • 27
    • 13
    • 11
    • 2
    • 2
    • 1
    Souris
    Clonalité
    • 65
    • 47
    Monoclonal
    Conjugué
    • 59
    • 12
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Podoplanin est conjugé à/à la Biotin
    Application
    • 74
    • 37
    • 34
    • 32
    • 20
    • 20
    • 16
    • 8
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificité
    This antibody reacts with Podoplanin (gp36). The unconjugated monoclonal antibody will detect native Human Podoplanin in Western Blot and on the surface of Human LEC.
    Réactivité croisée (Details)
    Species reactivity (tested):Human.
    Purification
    Affinity Chromatography on Protein G
    Immunogène
    Podoplanin-expressing MDCK cells.
    Clone
    18H5
    Isotype
    IgG1
    Top Product
    Discover our top product PDPN Anticorps primaire
  • Indications d'application
    Flow cytometry: use at 2-5 μg/mL together with the appropriate secondary reagents.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.
    Buffer
    PBS, 0.02 % Sodium Azide, BSA (50x)
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • Conrad, Niess, Huss, Huber, von Luettichau, Nelson, Ott, Jauch, Bruns: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." dans: Circulation, Vol. 119, Issue 2, pp. 281-9, (2009) (PubMed).

    Turner, Langley, Johnston, Gentil, Ford, Wu, Graham, Sharpley, Slatko, Pearlman, Taylor: "Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis." dans: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22364-78, (2009) (PubMed).

    Kilic, Oliveira-Ferrer, Neshat-Vahid, Irmak, Obst-Pernberg, Wurmbach, Loges, Kilic, Weil, Lauke, Tilki, Singer, Ergün: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." dans: Blood, Vol. 110, Issue 13, pp. 4223-33, (2007) (PubMed).

  • Antigène
    Podoplanin (PDPN)
    Autre désignation
    Podoplanin (PDPN Produits)
    Synonymes
    anticorps PDPN, anticorps AGGRUS, anticorps GP36, anticorps GP40, anticorps Gp38, anticorps HT1A-1, anticorps OTS8, anticorps PA2.26, anticorps T1A, anticorps T1A-2, anticorps OTS-8, anticorps RANDAM-2, anticorps T1-alpha, anticorps T1a, anticorps T1alpha, anticorps E11, anticorps RTI40, anticorps podoplanin, anticorps PDPN, anticorps Pdpn
    Sujet
    Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha
    ID gène
    10630
    NCBI Accession
    NP_001006625
    UniProt
    Q86YL7
    Pathways
    Dicarboxylic Acid Transport
Vous êtes ici:
Support technique