You are viewing an incomplete version of our website. Please click to reload the website as full version.

CD4 anticorps (CD4 Molecule) (Internal Region)

Détail du produit anti-CD4 anticorps No. ABIN1687412, Fournisseur: Connectez-vous pour afficher Nouveau
Nouveau
Request Données supplémentaires souhaité pour un produit?

L'Initiative de validation indépendante s'efforce de vous fournir des données de haute qualité. Savoir plus

Antigène
Synonymes cd4, L3T4, Ly-4, CD4mut, W3/25, p55
Alternatives
Épitope
Internal Region
(68), (22), (21), (15), (13), (13), (13), (10), (8), (7), (7), (7), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité
Humain
(993), (680), (224), (64), (42), (31), (24), (21), (20), (15), (14), (9), (8), (7), (7), (7), (6), (5), (3), (3), (2), (2), (2), (2), (1), (1)
Hôte
Lapin
(1180), (637), (206), (4), (2), (1)
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp CD4 est non-conjugé
(278), (237), (176), (122), (38), (38), (28), (24), (21), (20), (19), (18), (16), (15), (14), (13), (10), (9), (8), (8), (7), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochimie (Coupes en paraffine) (IHC (p)), Cytométrie de flux (FACS)
(1529), (372), (337), (274), (212), (209), (174), (101), (99), (67), (33), (31), (29), (18), (14), (13), (11), (9), (8), (6), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 21 références disponible
Fournisseur Connectez-vous pour afficher Nouveau
Numéro du produit fournisseur Connectez-vous pour afficher Nouveau
Quantité 0.1 mL
Destination France ( )
Disponibilité Envoi sous 6 à 8 jours ouvrables
Immunogène Synthetic peptide corresponding to internal region of human CD4.
Clone SP35
Isotype IgG
Homologie Pig (Porcine)
Attributs du produit Cellular Localization: Membrane
Purification Purified by Protein A/G
Autre désignation CD4 (CD4 Antibody Extrait)
Sujet CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45 % of peripheral blood lymphocytes. It is also present on 80 % of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV and is expressed in T cell lymphomas. Non-specific nuclear staining is sometimes seen in follicles with this antibody, however in most cases it does not interfere with the interpretation of the CD4 membrane staining.
Poids moléculaire 59 kDa
ID gène 920
Domaine de recherche Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling
Indications d'application IHC Dilution: 1:50
Commentaires

Positive Control:
IHC (p): Tonsil, T Cell Lymphoma
FC: Jurkat cell line

Protocole IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Restrictions For Research Use only
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Conseil sur la manipulation Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Stock 4 °C
Supplier Images
Cytométrie de flux (FACS) image for anti-CD4 anticorps (CD4 Molecule) (Internal Region) (ABIN1687412) Flow cytometric analysis of rabbit anti-CD4 (SP35) antibody in Jurkats (green) compar...
Immunohistochimie (IHC) image for anti-CD4 anticorps (CD4 Molecule) (Internal Region) (ABIN1687412) anti-CD4 Molecule (CD4) (Internal Region) antibody (Image 2)
Immunohistochimie (IHC) image for anti-CD4 anticorps (CD4 Molecule) (Internal Region) (ABIN1687412) anti-CD4 Molecule (CD4) (Internal Region) antibody (Image 3)
Immunohistochimie (IHC) image for anti-CD4 anticorps (CD4 Molecule) (Internal Region) (ABIN1687412) anti-CD4 Molecule (CD4) (Internal Region) antibody (Image 4)
Produit citée dans: Guislain, Gadiot, Kaiser et al.: "Sunitinib pretreatment improves tumor-infiltrating lymphocyte expansion by reduction in intratumoral content of myeloid-derived suppressor cells in human renal cell carcinoma." dans: Cancer immunology, immunotherapy : CII, Vol. 64, Issue 10, pp. 1241-50, 2015 (PubMed).

Pino, Erkizia, Benet et al.: "HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells." dans: Retrovirology, Vol. 12, pp. 37, 2015 (PubMed).

Nishikori, Kitano, Kobayashi et al.: "Increased number of peripheral CD8+ T cells but not eosinophils is associated with late-onset skin reactions caused by bendamustine." dans: International journal of hematology, Vol. 102, Issue 1, pp. 53-8, 2015 (PubMed).

Lavotshkin, Jalas, Torisu-Itakura et al.: "Immunoprofiling for prognostic assessment of colon cancer: a novel complement to ultrastaging." dans: Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract, Vol. 19, Issue 6, pp. 999-1006, 2015 (PubMed).

Llosa, Cruise, Tam et al.: "The vigorous immune microenvironment of microsatellite instable colon cancer is balanced by multiple counter-inhibitory checkpoints." dans: Cancer discovery, Vol. 5, Issue 1, pp. 43-51, 2015 (PubMed).

Lemos, Lama, Karuna et al.: "The inner foreskin of healthy males at risk of HIV infection harbors epithelial CD4+ CCR5+ cells and has features of an inflamed epidermal barrier." dans: PLoS ONE, Vol. 9, Issue 9, pp. e108954, 2014 (PubMed).

Kragsnaes, Fredberg, Stribolt et al.: "Stereological quantification of immune-competent cells in baseline biopsy specimens from achilles tendons: results from patients with chronic tendinopathy followed for more than 4 years." dans: The American journal of sports medicine, Vol. 42, Issue 10, pp. 2435-45, 2014 (PubMed).

Carulli, Ottaviano, Guerri et al.: "Multiparameter Flow Cytometry to Detect Hematogones and to Assess B-lymphocyte clonality in Bone Marrow Samples from Patients with Non-Hodgkin Lymphomas." dans: Hematology reports, Vol. 6, Issue 2, pp. 5381, 2014 (PubMed).

Cogbill, Swerdlow, Gibson: "Utility of CD279/PD-1 immunohistochemistry in the evaluation of benign and neoplastic T-cell-rich bone marrow infiltrates." dans: American journal of clinical pathology, Vol. 142, Issue 1, pp. 88-98, 2014 (PubMed).

Zanelli, Valli, Capodanno et al.: "Anaplastic lymphoma kinase-positive large B-cell lymphoma: description of a case with an unexpected clinical outcome." dans: International journal of surgical pathology, Vol. 23, Issue 1, pp. 78-83, 2015 (PubMed).

Liu, De Marchi, Timmermans et al.: "Ferritin heavy chain in triple negative breast cancer: a favorable prognostic marker that relates to a cluster of differentiation 8 positive (CD8+) effector T-cell response." dans: Molecular & cellular proteomics : MCP, Vol. 13, Issue 7, pp. 1814-27, 2014 (PubMed).

Wick, Webb, Nielsen et al.: "Surveillance of the tumor mutanome by T cells during progression from primary to recurrent ovarian cancer." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 20, Issue 5, pp. 1125-34, 2014 (PubMed).

Mori, Hiroi, Shimada et al.: "Infiltration of m2 tumor-associated macrophages in oral squamous cell carcinoma correlates with tumor malignancy." dans: Cancers, Vol. 3, Issue 4, pp. 3726-39, 2013 (PubMed).

Quintanilla-Martinez, Ridaura, Nagl et al.: "Hydroa vacciniforme-like lymphoma: a chronic EBV+ lymphoproliferative disorder with risk to develop a systemic lymphoma." dans: Blood, Vol. 122, Issue 18, pp. 3101-10, 2013 (PubMed).

Yoshii, Ishida, Hodohara et al.: "Systemic Epstein-Barr virus-positive T-cell lymphoproliferative disease of childhood: Report of a case with review of the literature." dans: Oncology letters, Vol. 4, Issue 3, pp. 381-384, 2013 (PubMed).

Cimino-Mathews, Ye, Meeker et al.: "Metastatic triple-negative breast cancers at first relapse have fewer tumor-infiltrating lymphocytes than their matched primary breast tumors: a pilot study." dans: Human pathology, Vol. 44, Issue 10, pp. 2055-63, 2013 (PubMed).

Wilson, Swerdlow, Przybylski et al.: "Intestinal ?? T-cell lymphomas are most frequently of type II enteropathy-associated T-cell type." dans: Human pathology, Vol. 44, Issue 6, pp. 1131-45, 2013 (PubMed).

Ishida, Mochizuki, Saito et al.: "CD8(+) mycosis fungoides with esophageal involvement: A case report." dans: Oncology letters, Vol. 5, Issue 1, pp. 73-75, 2012 (PubMed).

Rigau, Mania, Béfort et al.: "Lethal multiple sclerosis relapse after natalizumab withdrawal." dans: Neurology, Vol. 79, Issue 22, pp. 2214-6, 2012 (PubMed).

Donson, Birks, Schittone et al.: "Increased immune gene expression and immune cell infiltration in high-grade astrocytoma distinguish long-term from short-term survivors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 4, pp. 1920-7, 2012 (PubMed).

Bak, Stenderup, Rosada et al.: "Targeting of human interleukin-12B by small hairpin RNAs in xenografted psoriatic skin." dans: BMC dermatology, Vol. 11, pp. 5, 2011 (PubMed).

N° du produit ABIN1687412
219,05 €
Plus frais de livraison 40,00 € et TVA

Contact commande:

  • +33 (0)1 76 63 32 65
  • +33 (0)1 74 18 08 22