Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

IL-8 anticorps

IL8 Reactivité: Humain WB, ELISA, IHC (p) Hôte: Souris Monoclonal unconjugated
N° du produit ABIN1724413
  • Antigène Voir toutes IL-8 (IL8) Anticorps
    IL-8 (IL8) (Interleukin 8 (IL8))
    Reactivité
    • 138
    • 22
    • 15
    • 13
    • 12
    • 12
    • 10
    • 9
    • 9
    • 6
    • 4
    • 4
    • 4
    • 3
    • 3
    • 1
    Humain
    Hôte
    • 119
    • 106
    • 7
    • 3
    • 3
    • 3
    • 1
    Souris
    Clonalité
    • 135
    • 107
    Monoclonal
    Conjugué
    • 127
    • 50
    • 29
    • 9
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Cet anticorp IL-8 est non-conjugé
    Application
    • 157
    • 102
    • 78
    • 36
    • 35
    • 33
    • 16
    • 16
    • 15
    • 14
    • 10
    • 9
    • 9
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificité
    Reacts with human IL-8
    Purification
    Purified
    Immunogène
    Recombinant protein corresponding to full length IL8
    Isotype
    IgG1
    Top Product
    Discover our top product IL8 Anticorps primaire
  • Indications d'application

    Working dilution: Optimal dilution should be determined by the end user.
    The following are guidelines only:
    IHC-P (5 μg/mL) WB (1:1000)

    Restrictions
    For Research Use only
  • Validation #100789 (Immunohistochemistry)
    'Independent Validation' signe
    by
    HIK Hannover
    No.
    #100789
    Date
    31.05.2017
    Antigène
    IL8
    Numéro du lot
    Application validée
    Immunohistochemistry
    Contrôle positif
    Formalin fixed and plastic embedded human liver, testicle, and tonsil tissue sections
    Contrôle négative
    No primary antibody
    Conclusion
    Passed. The IL8 antibody ABIN1724413 specifically labels the targeted antigen in human liver, testicle, and tonsil sections in IHC. No signal was detected in tonsil negative control tissue sections and the no primary antibody negative controls.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN1724413
    Anticorps secondaire
    Histofine Simple Stain MAX PO (M) Anti-Mouse (Medac Diagnostika, 414132F, lot no H1608)
    Full Protocol
    • Disect tissue and fix in 4% buffered formalin for >24h at RT.
    • Plastification in Technovit 9100 plastic embedding system (Heraeus Kulzer) according to manufactuer’s recommencations.
    • Sectioning into 5µm sections on a rotary microtome (Reichert Jung, 1140/Autocut).
    • Deplastify and rehydrate tissue through graded xylene and graded alcohol series:
      • xylene for 20min.
      • MEA (2-Methoxyethyl)acetat zur Synthese (Merck, 8.06061.1000) 2x 20min.
      • 100% ethanol for 1min.
      • 95% ethanol for 1min.
      • 70% ethanol for 1min.
      • H2O for 2min.
      • distilled H2O 2min.
    • Heat induced epitope retrieval in high pH Trilogy buffer (Medac Diagnostika, 920P-07, lot 1622912A) ON at 60°C in an oven (Memmert, UNB 200).
    • Quench sections in in ice cold distilled H2O 2min.
    • Adjust sections with wash buffer (Medac Diagnostika, B1-30A, lot no 161102001) 5min at RT.
    • Incubate sections with primary Interleukin 8 antibody (antibodies-online, ABIN1724413) diluted 1:400 in dilution buffer (Medac Diagnostika, B1-31C, lot no 161205001) for 30min at RT. Includeno primaryantibody negative controls.
    • Rinse sections 3x 5min with wash buffer. Keep negative controls in a separate container.
    • Block endogenous peroxidase with Peroxide Block RTU (Medac Diagnostika, 925B-09, lot no 161103001) for 10min at RT.
    • Rinse sections 3x 5min with wash buffer.
    • Incubate sections with Histofine Simple Stain MAX PO (M) Anti-Mouse (Medac Diagnostika, 414132F, lot no H1608) for 30min at RT.
    • Rinse sections 3x 5min with wash buffer.
    • Incubate sections with Bright DAB (Medac Diagnostika, BS04-110, lot no 160523) for 10min at RT.
    • Rinse sections in distilled H2O.
    • Counterstain with Mayers Haematoxylin (Leica, 3801582E, lot no3801582E) 5min at RT.
    • Dehydrate in fresh ethanol:
      • 95% ethanol twice for 2min.
      • 99.5% ethanol twice for 2min.
      • Xylene twice for 2min.
    • Mount sections with Celltexx mounting medium (Engelbrecht, 19360, lot no 1716047).
    • Dry sections in a ventilated place.
    • Image acquisition on a Leica Microsystems DM 1000 light microscope with a DFC 320 digital camera. Magnification tonsil sections 40x, liver sections 10x and 20x, testicle sections 10x.
    Notes
    Immunostaining pattern of human liver, testicle, and tonsil with ABIN1724413 is consistent with published data.
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Citrate-Tris-HCl buffer, pH 7.0, 0.02 % Proclin 300
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
  • Ingendoh-Tsakmakidis, Mikolai, Winkel, Szafrański, Falk, Rossi, Walles, Stiesch: "Commensal and pathogenic biofilms differently modulate peri-implant oral mucosa in an organotypic model." dans: Cellular microbiology, pp. e13078, (2019) (PubMed).

  • Antigène
    IL-8 (IL8) (Interleukin 8 (IL8))
    Autre désignation
    IL-8 (IL8 Produits)
    Synonymes
    anticorps CXCL8, anticorps GCP-1, anticorps GCP1, anticorps LECT, anticorps LUCT, anticorps LYNAP, anticorps MDNCF, anticorps MONAP, anticorps NAF, anticorps NAP-1, anticorps NAP1, anticorps IL-8, anticorps AMCF-I, anticorps il8, anticorps IL8, anticorps si:dkey-151b16.2, anticorps cxcli2, anticorps cxcl8, anticorps gcp-1, anticorps il-8, anticorps lynap, anticorps mdncf, anticorps monap, anticorps nap-1, anticorps C-X-C motif chemokine ligand 8, anticorps interleukin 8, anticorps chemokine (C-X-C motif) ligand 8a, anticorps chemokine (C-X-C motif) ligand 1-like, anticorps chemokine (C-X-C motif) ligand 8 L homeolog, anticorps CXCL8, anticorps il8, anticorps cxcl8a, anticorps IL8, anticorps Cxcl8, anticorps LOC422654, anticorps cxcl8.L
    Sujet
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
    ID gène
    3576
    Pathways
    Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
Vous êtes ici:
Support technique