GABRA3 anticorps (AA 29-43)

N° du produit ABIN1742459

Détail du produit anti-GABRA3 anticorps

Antigène: GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))

Épitope: AA 29-43

Reactivité: Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GABRA3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificité: Specific for GABA-A receptor alpha3.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogène: Synthetic peptide (aa 29-43 of rat GABA-A receptor alpha3 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.

Information d'application

Indications d'application: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500

Commentaires:

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Conseil sur la manipulation: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Stock: -20 °C

Stockage commentaire: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Références pour anticorps anti-GABRA3 (ABIN1742459)

Tzanoulinou, García-Mompó, Castillo-Gómez, Veenit, Nacher, Sandi: "Long-term behavioral programming induced by peripuberty stress in rats is accompanied by GABAergic-related alterations in the Amygdala." dans: PLoS ONE, Vol. 9, Issue 4, pp. e94666, (2014) (PubMed).

Détails sur GABRA3

Antigène: GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))

Autre désignation: GABA-A Receptor Alpha3 (GABRA3 Produits)

Synonymes: anticorps GABRA3, anticorps Gabra-3, anticorps si:ch211-113k9.1, anticorps gamma-aminobutyric acid type A receptor alpha3 subunit, anticorps gamma-aminobutyric acid (GABA) A receptor, alpha 3, anticorps gamma-aminobutyric acid (GABA) A receptor, subunit alpha 3, anticorps gamma-aminobutyric acid receptor subunit alpha-3, anticorps GABRA3, anticorps gabra3, anticorps Gabra3, anticorps LOC100653496

Sujet: Synonyms: GABA(A) R alpha3