V5 Epitope Tag anticorps

N° du produit ABIN1842466

Détail du produit anti-V5 Epitope Tag anticorps

Antigène: V5 Epitope Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp V5 Epitope Tag est non-conjugé

Application: Western Blotting (WB), ELISA

Séquence: GKPIPNPLLG LDST

Specificité: Rabbit Anti-V5-tag Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.

Attributs du produit: V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.

Purification: Immunoaffinity chromatography

Immunogène: V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors,
Including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0. 05-0. 2 µg/mL
Western blot: 0. 1-1. 0 µg/mL
Western blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit L00204, C is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane. Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.

Références pour anticorps anti-V5 Epitope Tag (ABIN1842466)

Afrazi, Sodhi, Good, Jia, Siggers, Yazji, Ma, Neal, Prindle, Grant, Branca, Ozolek, Chang, Hackam: "Intracellular heat shock protein-70 negatively regulates TLR4 signaling in the newborn intestinal epithelium." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 9, pp. 4543-57, (2012) (PubMed).

Détails sur V5 Epitope Tag

Antigène: V5 Epitope Tag

Abstract: V5 Epitope Tag Produits

Classe de substances: Tag

Sujet: The V5 epitope tag is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).