B-Cell Linker anticorps (C-Term)

N° du produit ABIN184602

Détail du produit anti-B-Cell Linker anticorps

Antigène: B-Cell Linker (BLNK)

Épitope: C-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp B-Cell Linker est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Fonction: BLNK / SLP-65

Séquence: KDSTRLKYAV KVS

Specificité: This antibody is expected to recognize all reported isoforms.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-KDSTRLKYAVKVS, from the C Terminus of the protein sequence according to NP_037446.1, NP_001107566.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 70-75 kDa band observed in lysates of cell line Daudi (calculated MW of 50.5 kDa according to NP_037446.1). This molecular weight is routinely observed by other sources. Recommended concentration 0.3-1 μg/mL. Primary incubation 1 hour a

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the membranes of HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur B-Cell Linker

Antigène: B-Cell Linker (BLNK)

Autre désignation: BLNK (BLNK Produits)

Synonymes: anticorps BLNK, anticorps blnk, anticorps MGC147045, anticorps BASH, anticorps Bca, anticorps Ly-57, anticorps Ly57, anticorps Lyw-57, anticorps SLP-65, anticorps AGM4, anticorps BLNK-S, anticorps LY57, anticorps SLP65, anticorps bca, anticorps B-cell linker, anticorps B cell linker, anticorps BLNK, anticorps blnk, anticorps Blnk

Sujet: BLNK, SLP-65, B-cell linker, BASH, BLNK-s, Ly57, MGC111051, SLP65, B cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, OTTHUMP00000020167, Src homology 2 domain-containing leuko

ID gène: 29760, 17060

NCBI Accession: NP_037446, NP_001107566, NP_001245369, NP_001245370, NP_001245371

Pathways: BCR Signaling