ARPC2 anticorps (C-Term)

N° du produit ABIN184748

Il existe 1 publication pour ce produit. L’anticorps anti-ARPC2 Polyclonal Chèvre est utilisé pour la détection de ARPC2 dans des échantillons de Humain. Il a été validé pour WB, ELISA, IHC et IF.

Aperçu rapide pour ARPC2 anticorps (C-Term) (ABIN184748)

Antigène: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ARPC2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-ARPC2 anticorps

Épitope: C-Term

Fonction: ARPC2

Séquence: EMKTITGKTF SSR

Specificité: The reported variants NP_005722.1 and NP_690601.1 represent identical protein.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-EMKTITGKTFSSR, from the C Terminus of the protein sequence according to NP_005722.1, NP_690601.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 34-35 kDa band observed in lysates of cell lines CACO-2, HeLa and Jurkat (calculated MW of 34.3 kDa according to NP_005722.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-ARPC2 (ABIN184748)

Sakata, Taniguchi, Yasuda, Adachi-Morishima, Hamazaki, Nakayama, Miki, Minato, Narumiya: "Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1." dans: The Journal of experimental medicine, Vol. 204, Issue 9, pp. 2031-8, (2007) (PubMed).

Détails sur ARPC2

Antigène: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Autre désignation: ARPC2

Sujet: ARPC2, actin related protein 2/3 complex, subunit 2, 34 kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34, actin related protein 2/3 complex subunit 2

ID gène: 10109, 76709

NCBI Accession: NP_005722, NP_690601

Pathways: Signalisation RTK, Regulation of Actin Filament Polymerization