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TRIM55 anticorps (C-Term)

Verified TRIM55 Reactivité: Humain WB, ELISA, IHC, FACS, IF Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN184953
  • Antigène Voir toutes TRIM55 Anticorps
    TRIM55 (Tripartite Motif Containing 55 (TRIM55))
    Épitope
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 18
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Humain
    Hôte
    • 16
    • 2
    Chèvre
    Clonalité
    • 17
    • 1
    Polyclonal
    Conjugué
    • 14
    • 2
    • 1
    • 1
    Cet anticorp TRIM55 est non-conjugé
    Application
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
    Fonction
    MURF2 / TRIM55
    Séquence
    HIFSFSWLNS LNE
    Specificité
    This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.).
     Réactivité croisée
    Chien, Humain, Souris, Porc, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence C-HIFSFSWLNSLNE, from the C Terminus of the protein sequence according to NP_908973.1, NP_908974.1, NP_908975.1.
    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.

    Western Blot: Approx 58 kDa band observed in lysates of cell line HeLa (calculated MW of 60.5 kDa according to NP_908973.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the Golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène
    TRIM55 (Tripartite Motif Containing 55 (TRIM55))
    Autre désignation
    TRIM55 (TRIM55 Produits)
    Synonymes
    anticorps MURF-2, anticorps RNF29, anticorps muRF2, anticorps trim55, anticorps wu:fb71c01, anticorps zgc:92123, anticorps TRIM55, anticorps im:7145460, anticorps zgc:136833, anticorps D830041C10Rik, anticorps Murf2, anticorps Rnf29, anticorps murf-2, anticorps rnf29, anticorps tripartite motif containing 55, anticorps tripartite motif containing 55a, anticorps tripartite motif containing 55b, anticorps tripartite motif-containing 55, anticorps tripartite motif containing 55 L homeolog, anticorps TRIM55, anticorps trim55a, anticorps trim55b, anticorps Trim55, anticorps trim55.L
    Sujet
    TRIM55, RNF29, MURF-2, ring finger protein 29, for muscle specific ring finger 2, tripartite motif-containing 55, muscle specific ring finger 2
    ID gène
    84675, 381485, 365751
    NCBI Accession
    NP_908973, NP_908974, NP_908975
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