AHI1 anticorps (N-Term)

N° du produit ABIN185303

Détail du produit anti-AHI1 anticorps

Antigène: AHI1 (Abelson Helper Integration Site 1 (AHI1))

Épitope: N-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp AHI1 est non-conjugé

Application: ELISA, Flow Cytometry (FACS)

Fonction: AHI1 / Jouberin

Séquence: PTAESEAKVK TC

Homologie: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence PTAESEAKVKTC, from the N Terminus of the protein sequence according to NP_060121.3, NP_001128303.1, NP_001128302.1, NP_001128304.1.

Isotype: IgG

Information d'application

Indications d'application:

DS WB Results: Preliminary experiments gave a consistent band at approx 75-80 kDa in Human Cerebellum and Frontal Cortex lysates after 0.5 μg/mL antibody staining and was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 137 kDa according to NP_060121.3

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-AHI1 (ABIN185303)

Kennah, Ringrose, Zhou, Esmailzadeh, Qian, Su, Zhou, Jiang: "Identification of tyrosine kinase, HCK, and tumor suppressor, BIN1, as potential mediators of AHI-1 oncogene in primary and transformed CTCL cells." dans: Blood, Vol. 113, Issue 19, pp. 4646-55, (2009) (PubMed).

Zhou, Zhao, Ringrose, DeGeer, Kennah, Lin, Sheng, Li, Turhan, Jiang: "AHI-1 interacts with BCR-ABL and modulates BCR-ABL transforming activity and imatinib response of CML stem/progenitor cells." dans: The Journal of experimental medicine, Vol. 205, Issue 11, pp. 2657-71, (2008) (PubMed).

Détails sur AHI1

Antigène: AHI1 (Abelson Helper Integration Site 1 (AHI1))

Autre désignation: AHI1 (AHI1 Produits)

Synonymes: anticorps AHI1, anticorps AHI-1, anticorps JBTS3, anticorps ORF1, anticorps dJ71N10.1, anticorps 1700015F03Rik, anticorps Ahi-1, anticorps D10Bwg0629e, anticorps Abelson helper integration site 1, anticorps AHI1, anticorps ahi1, anticorps Ahi1

Sujet: AHI1, AHI-1, DKFZp686J1653, FLJ14023, FLJ20069, JBTS3, ORF1, dJ71N10.1, Abelson helper integration site, jouberin, Ahi-1 isoform I, OTTHUMP00000017263, OTTHUMP00000017264, OTTHUMP00000017265, contatins SH3 and WD40 domains, Abelson helper integration site 1

ID gène: 54806

NCBI Accession: NP_060121, NP_001128304, NP_001337433