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PD-L1 anticorps (C-Term)

Verified PD-L1 Reactivité: Humain WB, FACS, ELISA, IF Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN185339
  • Antigène Voir toutes PD-L1 Anticorps
    PD-L1 (CD274 (PD-L1))
    Épitope
    • 29
    • 16
    • 15
    • 12
    • 11
    • 11
    • 8
    • 7
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    • 5
    • 4
    • 3
    • 2
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    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 169
    • 108
    • 63
    • 7
    • 6
    • 5
    • 5
    • 1
    • 1
    Humain
    Hôte
    • 126
    • 62
    • 17
    • 4
    • 3
    • 1
    Chèvre
    Clonalité
    • 107
    • 104
    • 2
    Polyclonal
    Conjugué
    • 97
    • 16
    • 13
    • 11
    • 9
    • 5
    • 5
    • 5
    • 5
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    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Cet anticorp PD-L1 est non-conjugé
    Application
    • 137
    • 103
    • 59
    • 40
    • 37
    • 28
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    Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)
    Fonction
    CD274 / PD-L1
    Séquence
    CKKQSDTHLE ET
    Specificité
    This antibody is expected to recognize reported isoforms a and b (NP_054862.1, NP_001254635.1) only.
     Réactivité croisée
    Humain
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence CKKQSDTHLEET, from the C Terminus of the protein sequence according to NP_054862.1.
    Isotype
    IgG
  • Indications d'application

    Western Blot: Approx 50 kDa band observed in Human Heart lysates and in lysates of cell line U2OS (calculated MW of 33.3 kDa according to 1NP_054862.1).This band was successfully blocked by incubation with the immunizing peptide and is routinely observed

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in th membrane and cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Chen, Li, Meng, Fan, Song, Wang, Zhu, Guo, Zhang, Shi: "Upregulation of B7-H1 expression is associated with macrophage infiltration in hepatocellular carcinomas." dans: Cancer immunology, immunotherapy : CII, Vol. 61, Issue 1, pp. 101-8, (2012) (PubMed).

  • Antigène
    PD-L1 (CD274 (PD-L1))
    Autre désignation
    CD274 (PD-L1 Produits)
    Synonymes
    anticorps B7-H, anticorps B7H1, anticorps PD-L1, anticorps PDCD1L1, anticorps PDCD1LG1, anticorps PDL1, anticorps A530045L16Rik, anticorps B7h1, anticorps Pdcd1l1, anticorps Pdcd1lg1, anticorps Pdl1, anticorps RGD1566211, anticorps CD274 molecule, anticorps CD274 antigen, anticorps programmed cell death 1 ligand 2, anticorps CD274, anticorps Cd274, anticorps PDCD1LG2
    Sujet
    CD274, CD274 antigen, PD-L1, PDCD1LG1, B7-H, B7H1, PDL1, PDCD1L1, programmed cell death 1 ligand 1, PDL1, HGNC:17635, CD274 Molecule, MGC142294, MGC142296
    ID gène
    29126
    NCBI Accession
    NP_054862, NP_001254635
    Pathways
    Cancer Immune Checkpoints
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