GATA1 anticorps (Internal Region)

N° du produit ABIN185449

Détail du produit anti-GATA1 anticorps

Antigène: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Épitope: Internal Region

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GATA1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: GATA1

Séquence: DAEAYRHSPV FQ

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-DAEAYRHSPVFQ, from the internal region of the protein sequence according to NP_002040.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 50-55 kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8 kDa according to NP_002040.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur GATA1

Antigène: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Autre désignation: GATA1 (GATA1 Produits)

Synonymes: anticorps ERYF1, anticorps GATA-1, anticorps GF-1, anticorps GF1, anticorps NF-E1, anticorps NFE1, anticorps XLANP, anticorps XLTDA, anticorps XLTT, anticorps Gata-1, anticorps Gf-1, anticorps eryf1, anticorps Eryf1, anticorps GATA1, anticorps gata-1, anticorps Gata1, anticorps si:dkeyp-77f7.5, anticorps gata1, anticorps gata1a-A, anticorps xGATA-1, anticorps xgata1, anticorps XGATA-1B, anticorps gata1b-A, anticorps gta1, anticorps zg1, anticorps NF-E1a, anticorps GATA binding protein 1, anticorps GATA binding protein 1 (globin transcription factor 1), anticorps amidase, anticorps aspartyl/glutamyl-tRNA amidotransferase subunit A, anticorps putative amidase, anticorps GatA1, anticorps glutamyl-tRNA amidotransferase subunit A, anticorps hematopietic transcription factor GATA-1, anticorps GATA binding protein 1b, anticorps GATA binding protein 1 L homeolog, anticorps GATA binding protein 1 S homeolog, anticorps GATA binding protein 1a, anticorps erythroid transcription factor, anticorps GATA1, anticorps Gata1, anticorps gatA1, anticorps gata-1, anticorps gata1b, anticorps gata1.L, anticorps gata1.S, anticorps gata1a, anticorps LOC108348091

Sujet: GATA1, GATA binding protein 1 (globin transcription factor 1), HGNC:4170, ERYF1, GF1, NFE1, GATA binding protein 1, GATA-binding protein 1 (globin transcription factor 1), erythrold transcription factor 1, globin transcription factor 1, transcription fact

ID gène: 2623

NCBI Accession: NP_002040

Pathways: Cellular Response to Molecule of Bacterial Origin