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GAPDH anticorps (Internal Region)

Verified GAPDH Reactivité: Humain, Souris, Rat WB, ELISA, IHC, IF Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN185543
  • Antigène Voir toutes GAPDH Anticorps
    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
    Épitope
    • 37
    • 21
    • 13
    • 9
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivité
    • 222
    • 141
    • 134
    • 75
    • 65
    • 55
    • 36
    • 28
    • 23
    • 21
    • 20
    • 19
    • 18
    • 16
    • 12
    • 12
    • 11
    • 10
    • 9
    • 8
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 170
    • 97
    • 16
    • 10
    • 4
    Chèvre
    Clonalité
    • 190
    • 105
    Polyclonal
    Conjugué
    • 156
    • 36
    • 23
    • 17
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp GAPDH est non-conjugé
    Application
    • 243
    • 97
    • 83
    • 69
    • 46
    • 41
    • 41
    • 35
    • 16
    • 16
    • 15
    • 15
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
    Fonction
    GAPDH (Internal)
    Séquence
    GVNHEKYDNS LK
    Specificité
    This antibody is expected to recognize both reported isoforms (NP_002037.2, NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels
     Réactivité croisée
    Chien, Humain, Souris, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2.
    Isotype
    IgG
    Top Product
    Discover our top product GAPDH Anticorps primaire
  • Indications d'application

    Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2 μg/mL.

    Western Blot: Approx 37 kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1 kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Ramsden, Nommiste, R Lane, Carr, Powner, J K Smart, Chen, Muthiah, Webster, Moore, Cheetham, da Cruz, Coffey: "Rescue of the MERTK phagocytic defect in a human iPSC disease model using translational read-through inducing drugs." dans: Scientific reports, Vol. 7, Issue 1, pp. 51, (2019) (PubMed).

    Carr, Vugler, Yu, Semo, Coffey, Moss, Greenwood: "The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide." dans: Molecular vision, Vol. 17, pp. 1701-15, (2011) (PubMed).

    Carr, Vugler, Lawrence, Chen, Ahmado, Chen, Semo, Gias, da Cruz, Moore, Walsh, Coffey: "Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay." dans: Molecular vision, Vol. 15, pp. 283-95, (2009) (PubMed).

    Chakir, Daya, Aiba, Tunin, Dimaano, Abraham, Jaques-Robinson, Jacques, Lai, Pacak, Zhu, Xiao, Tomaselli, Kass: "Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy." dans: Circulation, Vol. 119, Issue 9, pp. 1231-40, (2009) (PubMed).

    Schillace, Miller, Pisenti, Grotzke, Swarbrick, Lewinsohn, Carr: "A-kinase anchoring in dendritic cells is required for antigen presentation." dans: PLoS ONE, Vol. 4, Issue 3, pp. e4807, (2009) (PubMed).

    Vugler, Carr, Lawrence, Chen, Burrell, Wright, Lundh, Semo, Ahmado, Gias, da Cruz, Moore, Andrews, Walsh, Coffey: "Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation." dans: Experimental neurology, Vol. 214, Issue 2, pp. 347-61, (2008) (PubMed).

  • Antigène
    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
    Autre désignation
    GAPDH (GAPDH Produits)
    Synonymes
    anticorps G3PD, anticorps GAPD, anticorps Gapd, anticorps BEST:GH12586, anticorps CG12055, anticorps Dmel\\CG12055, anticorps GA3PDH, anticorps GADPH, anticorps GAP, anticorps GAPDH, anticorps GAPDH I, anticorps GAPDH-1, anticorps GAPDH1, anticorps GAPDHI, anticorps Gapdh, anticorps Gapdh-1, anticorps Gapdh43E, anticorps gadph, anticorps gapdh, anticorps gapdh-1, anticorps gh12586, anticorps cb609, anticorps gapd, anticorps mg:bb02e05, anticorps wu:fb33a10, anticorps wu:ft80f05, anticorps KNC-NDS6, anticorps g3pd, anticorps G3PDH, anticorps glyceraldehyde-3-phosphate dehydrogenase, anticorps Glyceraldehyde 3 phosphate dehydrogenase 1, anticorps glyceraldehyde-3-phosphate dehydrogenase S homeolog, anticorps glyceraldehyde-3-phosphate dehydrogenase, type I, anticorps olfactory receptor 8K3, anticorps GAPDH, anticorps Gapdh, anticorps Gapdh1, anticorps gapdh, anticorps gapdh.S, anticorps gapDH, anticorps LOC100404960, anticorps LOC614985
    Sujet
    GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
    ID gène
    2597, 14433, 24383
    NCBI Accession
    NP_002037, NP_001243728
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