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EN2 anticorps (C-Term)

Verified EN2 Reactivité: Humain, Souris WB, ELISA, IF Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN185576
  • Antigène Voir toutes EN2 Anticorps
    EN2 (Engrailed Homeobox 2 (EN2))
    Épitope
    • 18
    • 15
    • 12
    • 5
    • 1
    C-Term
    Reactivité
    • 56
    • 33
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain, Souris
    Hôte
    • 54
    • 2
    Chèvre
    Clonalité
    • 54
    • 2
    Polyclonal
    Conjugué
    • 23
    • 6
    • 5
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp EN2 est non-conjugé
    Application
    • 50
    • 30
    • 13
    • 13
    • 9
    • 8
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    Fonction
    Engrailed-2
    Séquence
    NHSTTAKEGK SDSE
     Réactivité croisée
    Chien, Humain, Souris, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence C-NHSTTAKEGKSDSE, from the C Terminus of the protein sequence according to NP_001418.2.
    Isotype
    IgG
    Top Product
    Discover our top product EN2 Anticorps primaire
  • Indications d'application

    Western Blot: Approx 35 kDa band observed in Mouse Brain lysates (calculated MW of f 33.8 kDa according to Mouse NP_034264.1). Recommended concentration: 0.1-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:4000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the nuclei of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène
    EN2 (Engrailed Homeobox 2 (EN2))
    Autre désignation
    EN2 (EN2 Produits)
    Synonymes
    anticorps EN2, anticorps en, anticorps en2, anticorps BB131122, anticorps En-2, anticorps RGD1561842, anticorps Gg-En-2, anticorps engrailed homeobox 2, anticorps engrailed homeobox, anticorps engrailed 2, anticorps EN2, anticorps en, anticorps En2
    Sujet
    Engrailed-2, engrailed homolog 2 , EN2, AUTS1
    ID gène
    2020
    NCBI Accession
    NP_001418
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