Hsc70 anticorps (Internal Region)

N° du produit ABIN185659

Détail du produit anti-Hsc70 anticorps

Antigène: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Épitope: Internal Region

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Hsc70 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Fonction: HSPA8 (Isoform 1)

Séquence: EKLQGKINDE DKQK

Specificité: This antibody is expected to recognise isoform 1 (NP_006588.1) only.

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9 kDa according to Human NP_006588.1 and Mouse NP_112442.2). This molecular weight is routinely observed by other sources. Recommended concentration:

Peptide ELISA: antibody detection limit dilution 1:4000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-Hsc70 (ABIN185659)

Johnson, Kaplitt: "Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders." dans: PLoS ONE, Vol. 4, Issue 9, pp. e7100, (2009) (PubMed).

Détails sur Hsc70

Antigène: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Autre désignation: HSPA8 (HSPA8 Produits)

Sujet: HSPA8 , heat shock 70 kDa protein 8 , HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP, MGC131511, MGC29929, NIP71 , LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, heat shock 70kD protein 8, heat sh

ID gène: 3312, 15481, 24468

NCBI Accession: NP_006588