Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CA12 anticorps (AA 161-318)

CA12 Reactivité: Souris WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1858202
  • Antigène Voir toutes CA12 Anticorps
    CA12 (Carbonic Anhydrase 12 (CA12))
    Épitope
    • 15
    • 8
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 161-318
    Reactivité
    • 48
    • 8
    • 7
    Souris
    Hôte
    • 37
    • 10
    • 2
    Lapin
    Clonalité
    • 40
    • 9
    Polyclonal
    Conjugué
    • 26
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CA12 est non-conjugé
    Application
    • 30
    • 22
    • 13
    • 13
    • 11
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Fonction
    Polyclonal Antibody to Carbonic Anhydrase XII (CA12)
    Specificité
    The antibody is a rabbit polyclonal antibody raised against CA12. It has been selected for its ability to recognize CA12 in immunohistochemical staining and western blotting.
     Réactivité croisée
    Humain, Rat
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogène
    Recombinant Carbonic Anhydrase XII (CA12)
    Isotype
    IgG
    Top Product
    Discover our top product CA12 Anticorps primaire
  • Indications d'application

    Western blotting: 0.5-2 μg/mL

    1:500-2000 Immunohistochemistry: 5-20 μg/mL

    1:50-200 Immunocytochemistry: 5-20 μg/mL

    1:50-200 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Validation #100909 (Immunohistochemistry)
    'Independent Validation' signe
    by
    School of Clinical Sciences, University of Bristol
    No.
    #100909
    Date
    21.02.2017
    Antigène
    CA12
    Numéro du lot
    A20170109527
    Application validée
    Immunohistochemistry
    Contrôle positif
    mouse kidney, mouse ciliary body
    Contrôle négative
    secondary antibody only
    Conclusion
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN3204699
    Anticorps secondaire
    goat anti-rabbit IgG Alexa Fluor 488 antibody (Life Technology, A-11006, lot 1348202)
    Full Protocol
    • Cryo-section:
      • Wash mouse eye and kidney tissue in PBS.
      • Cryo-protection. Suspend tissue in Optimum Cutting Temperature (OCT) embedding media (Thermo Scientific, Lot 03801137).
      • Gently freeze suspended tissue in liquid nitrogen for 5min.
      • Sections performed on a cryostat (LEICA, CM3050S) set to 12µm.
      • Fix sections in 4% paraformaldehyde (PFA) for 5min at room temperature.
      • Rinse sections in PBS 3 times for 5min.
      • Block sections with 5% normal goat serum for 1h at RT.
    • Immunostaining:
      • Incubate sections with primary rabbit-anti CA12 antibody (antibodies-online, ABIN3204699, lot A20170109527) diluted 1:100 in PBSA, overnight at 4°C. Include a negative control without primary antibody and a kidney positive control.
      • Rinse sections with PBS 3 times for 5min. Keep negative control in a separate jar.
      • Incubate sections with secondary goat anti-rabbit IgG Alexa Fluor 488 antibody conjugate (Life Technology, A-11006, lot 1348202) diluted 1:500 in 1% Bovine Serum Albumin in PBS (PBSA) buffer for 2h at RT.
      • Counterstain with DAPI in the mounting medium step.
    • Wash and mount:
      • Rinse sections in PBS 3 times for 5min.
      • Mount sections in Vectorshield mounting medium with DAPI (Vector laboratories, H-1200, lot ZC0814).
    • Microscopy:
      • Image acquisition on an EVOS FL Color fluorescence and transmitted light microscope (Life technologies, Cat no. AMEFC4300) at 20x magnification.
    Notes
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Date de péremption
    24 months
  • Antigène
    CA12 (Carbonic Anhydrase 12 (CA12))
    Autre désignation
    Carbonic Anhydrase XII (CA12 Produits)
    Synonymes
    anticorps CAXII, anticorps HsT18816, anticorps CAR12, anticorps 2310047E01Rik, anticorps AI314958, anticorps Ca12, anticorps ca12, anticorps carbonic anhydrase 12, anticorps carbonic anhydrase XII L homeolog, anticorps CA12, anticorps Car12, anticorps ca12.L
    Sujet
    CA-XII, CAXII, Carbonate dehydratase XII, Tumor antigen HOM-RCC-3.1.3
Vous êtes ici:
Support technique