PANIER
AIDE
MONTRER AIDE VIDEOMontrer la vidéo expliquant commment utiliser le moteur de recherche
(Flash nécessaire)
Montrer la vidéo expliquant commment utiliser le moteur de recherche
(Flash nécessaire)
(Flash nécessaire)
Montrer la vidéo expliquant commment utiliser le moteur de recherche (Flash nécessaire)
Détail du produit
anti-P-Glycoprotein anticorps
Aperçu
| Antigène: | P-Glycoprotein |
| Clonalité: | Monoclonal (MRK16) |
| Application: | Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF) |
| Reactivité: | Human |
| Hôte: | Mouse |
|
2 références disponible |
| Quantité: | |
| Prix: | 570,00 $ (Plus frais de livraison $25 et TVA, Invoice in EUR) |
| Numéro du produit: | ABIN187222 |
| Disponibilité: | Envoi sous 7 à 10 jours ouvrables |
Description du produit » anti-P-Glycoprotein anticorps
| Antigène | P-Glycoprotein |
| ID gène | 5243 |
| Reactivité | Human |
| Clonalité | Monoclonal |
| Format | Purified |
| Isotype | IgG2a »Anticorps secondaires correspondants |
| Description | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq] Also known as: CLCS, MDR1, P-GP, PGY1, ABC20, CD243, GP170, MGC163296, ABCB1. |
| Clone | MRK16 |
| Hôte | Mouse |
Applications » anti-P-Glycoprotein anticorps
| Application | Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF) |
| Indications d'application | Potential Uses: cancer multidrug resistance studies, anti-cancer agent screening, AIDS multi-drug resistance studies, nervous system research, immunology research and transplant rejection studies, endocrine/steroid research, endothelium cell and brain P-glycoprotein research. Flow cytometry: For 5 x 105 pelleted cells use 50 µL of 10-100 µg/mL. Immunoprecipitation: Generally use between 25 µg/mL and 50 µg/mL. Immunohistochemistry: Generally use between 0.1 µg/mL and 10 µg/mL. Appropriate dilution must be decided upon according to staining method, conditions, etc. Frozen tissue staining can be done provided that mild fixation treatment is used, such as 4% paraformaldehyde. Acetone, paraffin, methyl alcohol, and ethyl alcohol should not be used as a fixative. Iodine labeling can also be performed on MRK16. Western blot: Does not react. Apparently, the epitope recognized by this antibody is lost in the presence of protein denaturing agents such as SDS. The optimal dilution for a specific application should be determined by the researcher. |
| Buffer | 300 µL of 500 µg/mL monoclonal antibody in PBS, with protein stabilizer, 0.1% sodium azide. Purified by Protein A affinity chromatography. Page 1 of 2 |
| Stock | Store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Restrictions | For Research Use only |
Références » anti-P-Glycoprotein anticorps
| Références |
de Figueiredo-Pontes, Pintao, Oliveira et al.: "Determination of P-glycoprotein, MDR-related protein 1, breast cancer resistance protein, and lung-resistance protein expression in leukemic stem cells of acute myeloid leukemia." In: Cytometry. Part B, Clinical cytometry
, Vol. 74, Issue 3, pp. 163-8, 2008 (PubMed)
Valera, de Freitas Cortez, de Paula Queiroz et al.: "Pediatric glioblastoma cell line shows different patterns of expression of transmembrane ABC transporters after in vitro exposure to vinblastine." In: Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery , Vol. 25, Issue 1, pp. 39-45, 2008 (PubMed) |
Antigènes
