CDKN1B anticorps (pThr187)

N° du produit ABIN196946

Détail du produit anti-CDKN1B anticorps

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Épitope: pThr187

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDKN1B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: P27Kip1 antibody detects endogenous levels of p27Kip1 only when phosphorylated at threonine 187.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: The antiserum was produced against synthesized phosphopeptide derived from human p27Kip1 around the phosphorylation site of threonine 187 (E-Q-TP-P-K).

Information d'application

Indications d'application: Western Blot: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS((without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur CDKN1B

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Autre désignation: CDKN1B / KIP1 (CDKN1B Produits)

Synonymes: anticorps CDKN4, anticorps KIP1, anticorps MEN1B, anticorps MEN4, anticorps P27KIP1, anticorps cdkn1b, anticorps sb:cb611, anticorps wu:fb63g10, anticorps wu:fb64g10, anticorps wu:fe18e03, anticorps CDKN1B, anticorps Kip1, anticorps p27, anticorps FAM14D, anticorps ISG12, anticorps ISG12A, anticorps P27, anticorps Cdki1b, anticorps AA408329, anticorps AI843786, anticorps p27Kip1, anticorps cdkn1bl, anticorps cyclin dependent kinase inhibitor 1B, anticorps cyclin-dependent kinase inhibitor 1Bb, anticorps interferon alpha inducible protein 27, anticorps cyclin-dependent kinase inhibitor 1B, anticorps cyclin-dependent kinase inhibitor 1Ba, anticorps CDKN1B, anticorps cdkn1bb, anticorps IFI27, anticorps Cdkn1b, anticorps cdkn1ba

Sujet: Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1, while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

ID gène: 1027

NCBI Accession: NP_004055

UniProt: P46527

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy