EIF2AK2 anticorps (pThr451)

N° du produit ABIN197058

Détail du produit anti-EIF2AK2 anticorps

Antigène: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Épitope: pThr451

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF2AK2 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: PKR antibody detects endogenous levels of PKR only when phosphorylated at threonine 451.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: The antiserum was produced against synthesized phosphopeptide derived from human PKR around the phosphorylation site of threonine 451 (K-G-TP-L-R).

Information d'application

Indications d'application: Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur EIF2AK2

Antigène: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Autre désignation: EIF2AK2 / PKR (EIF2AK2 Produits)

Synonymes: anticorps PKR, anticorps pkr, anticorps EIF2AK2, anticorps DKFZp469K1934, anticorps EIF2AK1, anticorps PRKR, anticorps 2310047A08Rik, anticorps 4732414G15Rik, anticorps AI467567, anticorps AI747578, anticorps Pkr, anticorps Prkr, anticorps Tik, anticorps eukaryotic translation initiation factor 2 alpha kinase 2, anticorps eukaryotic translation initiation factor 2-alpha kinase 2, anticorps EIF2AK2, anticorps eif2ak2, anticorps Eif2ak2

Sujet: PKR is an interferon-inducible serine/threonine specific protein kinase. It is widely expressed in eukaryotic organisms and activated by double stranded RNA. Activation of PKR by dsRNAs leads to autophosphorylation at multiple sites. Phosphorylation of Thr446 and Thr451 in the PKR activation loop is required in vivo and in vitro for high level kinase activity. PKR phosphorylates its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor 2 (EIF2 alpha), leading to the inhibition of protein synthesis. PKR is also involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, and also activates IKK and NFKB, thereby suppressing apoptosis. Recently, it has been reported that PKR also phosphorylates human p53 on serine 392. PKR might play a role in ER stress-induced apoptosis and in Alzheimer's disease. Alzheimer cases show prominent PKR activation in association with neuritic plaques and pyramidal neurons in the hippocampus and neocortex.Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-induced double-stranded RNA-activated protein kinase, PRKR, Protein kinase RNA-activated, eIF-2A protein kinase 2, p68 kinase

ID gène: 5610

NCBI Accession: NP_001129123

UniProt: P19525

Pathways: Réparation de l'ADN, ER-Nucleus Signaling, Hepatitis C