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APP anticorps (AA 666-670)

APP Reactivité: Humain, Souris, Rat WB, IF Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN197432
  • Antigène Voir toutes APP Anticorps
    APP (Amyloid beta (A4) Precursor Protein (APP))
    Épitope
    • 30
    • 28
    • 24
    • 24
    • 21
    • 18
    • 12
    • 8
    • 8
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 666-670
    Reactivité
    • 274
    • 120
    • 113
    • 13
    • 10
    • 10
    • 9
    • 9
    • 9
    • 8
    • 8
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 236
    • 72
    • 5
    • 5
    • 1
    Lapin
    Clonalité
    • 256
    • 63
    Polyclonal
    Conjugué
    • 148
    • 31
    • 23
    • 23
    • 12
    • 11
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Cet anticorp APP est non-conjugé
    Application
    • 212
    • 158
    • 93
    • 52
    • 52
    • 51
    • 41
    • 40
    • 30
    • 26
    • 16
    • 11
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Specificité
    This antibody detects endogenous levels of total APP protein.
    Purification
    Affinity Chromatography using epitope-specific immunogen.
    Immunogène
    peptide Sequence around amino acids 666~670 (A-V-T-P-E) derived from Human APP protein.
    Top Product
    Discover our top product APP Anticorps primaire
  • Indications d'application
    Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Validation #029576 (Immunofluorescence)
    'Independent Validation' signe
    by
    Molecular Pathology Core
    No.
    #029576
    Date
    11.01.2014
    Antigène
    Numéro du lot
    8715
    Application validée
    Immunofluorescence
    Contrôle positif
    Human brain
    Contrôle négative
    Human liver
    Conclusion
    Signal was detected in positive control tissue, and no signal was seen in negative control tissue.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    • Antibody: Amyloid beta A4 precursor protein (APP)
    • Catalog number: ABIN197433
    • Lot number: 8715
    Anticorps secondaire
    • Antibody: AlexaFluor 488 goat anti-Rabbit IgG
    • Lot number: 702323
    Full Protocol
    • Sections were deparaffinized and rehydrated.
    • Sections were heated to 98°C for 20 min in citrate buffer pH 6.0 (Biogenex, HK086-9K) for antigen retrieval and cooled down for 20 min on the bench.
    • Sections were blocked in 10% NGS (normal goat serum) for 20 min at room temperature.
    • Sections were washed x 2 in 1xTBS buffer.
    • Sections were incubated with primary antibody diluted 1:100 in Antibody Diluent (Invitrogen, 003218) at 4°C overnight.
    • Sections were washed x 2 in 1xTBS buffer.
    • Sections were incubated with AlexaFluor 488 Goat anti-Rabbit IgG 1:1000 for 60 min.
    • Sections were washed x 3 in 1xTBS buffer.
    • Sections were mounted with DAPI (Invitrogen, Prolong Gold antifade reagent with DAPI) and coverslipped.
    • Sections were photographed with a Zeiss Axioskop2 microscope
    Notes
    None
  • Concentration
    1.0 mg/mL
    Buffer
    PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Store the antibody (in aliquots) at -20 °C.
  • Antigène
    APP (Amyloid beta (A4) Precursor Protein (APP))
    Autre désignation
    Amyloid beta A4 Protein / APP (APP Produits)
    Synonymes
    anticorps AAA, anticorps ABETA, anticorps ABPP, anticorps AD1, anticorps APPI, anticorps CTFgamma, anticorps CVAP, anticorps PN-II, anticorps PN2, anticorps aaa, anticorps abeta, anticorps abpp, anticorps ad1, anticorps appi, anticorps ctfgamma, anticorps cvap, anticorps pn2, anticorps APP, anticorps APP-like, anticorps APPL, anticorps Abeta, anticorps BcDNA:GH04413, anticorps CG7727, anticorps Dmel\\CG7727, anticorps EG:65F1.5, anticorps appl, anticorps Abpp, anticorps Adap, anticorps Ag, anticorps Cvap, anticorps E030013M08Rik, anticorps betaApp, anticorps app, anticorps wu:fj34d10, anticorps wu:fk65e12, anticorps zgc:85740, anticorps amyloid beta precursor protein, anticorps amyloid beta (A4) precursor protein, anticorps beta amyloid protein precursor-like, anticorps amyloid beta (A4) precursor protein a, anticorps amyloid beta precursor protein L homeolog, anticorps APP, anticorps app, anticorps Appl, anticorps App, anticorps appa, anticorps app.L
    Sujet
    Amyloid beta precursor protein gene (ABPP) encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Multiple transcript variants encoding several different isoforms have been found for this gene. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in nonneuronal cells. Isoform APP751 is the most abundant form in T lymphocytes. ABPP is expressed in all fetal tissues examined with the highest levels in brain, kidney, heart and spleen with weak expression observed in liver, ABPP is induced during neuronal differentiation. In the adult brain, highest expression of ABPP gene is found in the frontal lobe of the cortex and in the anterior perisylvian cortex opercular gyri, moderate expression in the cerebellar cortex, the posterior perisylvian cortex opercular gyri and the temporal associated cortex. Weak expression is found in the striate, extra striate and motor cortices. Mutations in ABPP have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).Synonyms: ABPP, APPI, Alzheimer disease amyloid protein, Amyloid Precursor Protein, CVAP, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II
    ID gène
    351
    NCBI Accession
    NP_000475
    UniProt
    P05067
    Pathways
    Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour
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