Arylsulfatase A anticorps (Internal Region)

N° du produit ABIN238536

Détail du produit anti-Arylsulfatase A anticorps

Antigène: Arylsulfatase A (ARSA)

Épitope: Internal Region

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Arylsulfatase A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Fonction: Arylsulfatase A

Séquence: YDLSKDPGEN YN

Réactivité croisée: Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-YDLSKDPGENYN, from the internal region of the protein sequence according to NP_000478.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex) and Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60-65Da band observed in Mouse and Rat Testis lysates (calculated MW of 53.7 kDa according to Mouse NP_033843.2 and Rat NP_001030105.2,). This molecular weight is routinely observed by other sources and was successfully blocked by inc

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-Arylsulfatase A (ABIN238536)

Kanbe, Kamijo, Nakajima, Tanaka, Sugiyama, Wang, Fang, Hara, Gonzalez, Aoyama: "Chronic ethanol consumption decreases serum sulfatide levels by suppressing hepatic cerebroside sulfotransferase expression in mice." dans: Archives of toxicology, Vol. 88, Issue 2, pp. 367-79, (2014) (PubMed).

Kimura, Nakajima, Kamijo, Tanaka, Wang, Hara, Sugiyama, Tanaka, Gonzalez, Aoyama: "Hepatic Cerebroside Sulfotransferase Is Induced by PPAR? Activation in Mice." dans: PPAR research, Vol. 2012, pp. 174932, (2012) (PubMed).

Kiebish, Young, Lehman, Han: "Chronic caloric restriction attenuates a loss of sulfatide content in PGC-1?-/- mouse cortex: a potential lipidomic role of PGC-1? in neurodegeneration." dans: Journal of lipid research, Vol. 53, Issue 2, pp. 273-81, (2012) (PubMed).

Zhang, Nakajima, Kamijo, Li, Hu, Kannagi, Kyogashima, Aoyama, Hara: "Acute kidney injury induced by protein-overload nephropathy down-regulates gene expression of hepatic cerebroside sulfotransferase in mice, resulting in reduction of liver and serum sulfatides." dans: Biochemical and biophysical research communications, Vol. 390, Issue 4, pp. 1382-8, (2009) (PubMed).

Détails sur Arylsulfatase A

Antigène: Arylsulfatase A (ARSA)

Autre désignation: ARSA (ARSA Produits)

Synonymes: anticorps ARSA, anticorps zgc:101575, anticorps arsa, anticorps AS-A, anticorps ASA, anticorps AW212749, anticorps As-2, anticorps As2, anticorps TISP73, anticorps MLD, anticorps mld, anticorps arylsulfatase A, anticorps arylsulfatase, anticorps arylsulfatase A, gene 1 S homeolog, anticorps ARSA, anticorps arsa, anticorps arsA, anticorps RB6599, anticorps Arsa, anticorps arsa.1.S

Sujet: ARSA, arylsulfatase A, MLD, cerebroside-sulfatase, cerebroside 3-sulfatase

ID gène: 410, 11883, 315222

NCBI Accession: NP_000478