XTH-Xet anticorps

N° du produit ABIN249338

Détail du produit

Antigène: XTH-Xet

Reactivité: Orge, Oryza sativa

Hôte: Lapin

Clonalité: Polyclonal

Application: ELISA, Western Blotting (WB)

Réactivité croisée (Details): Not reactive in: Zea mays

Homologie: monocots

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 31.5 / 33 kDa

Purification: serum

Immunogène: two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet

Information d'application

Indications d'application: 1: 500 with standard ECL (WB) , 1: 5000 (ELISA)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Référence

Hrmova, Farkas, Lahnstein, Fincher: "A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans." dans: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12951-62, (2007) (PubMed).

Détail du antigène

Antigène: XTH-Xet

Sujet: Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3,1,4)-β-d-glucans, are fundamental to cell wall properties. These interactions have been assumed to be non-covalent in nature in most cases. A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3,1,4)-β-d-glucans. It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.

Poids moléculaire: expected: 31.5 kDa, apparent: 33 kDa