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Hsc70 anticorps

HSPA8 Reactivité: Humain, Rat, Souris, Boeuf (Vache), Poulet, Hamster, Chien, Mouton, Lapin, Porc, Cobaye, Drosophila melanogaster, Xenopus laevis, Saccharomyces cerevisiae, Poisson, Beluga, Pinctada fucata (Pearl oyster), Coquille Saint-Jacques, Artemia sp. (Brine shrimp) WB, IP, IF, IHC (fro) Hôte: Souris Monoclonal BB70 unconjugated
N° du produit ABIN263941
  • Antigène Voir toutes Hsc70 (HSPA8) Anticorps
    Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
    Reactivité
    • 84
    • 46
    • 42
    • 21
    • 17
    • 14
    • 13
    • 11
    • 11
    • 10
    • 10
    • 10
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Rat, Souris, Boeuf (Vache), Poulet, Hamster, Chien, Mouton, Lapin, Porc, Cobaye, Drosophila melanogaster, Xenopus laevis, Saccharomyces cerevisiae, Poisson, Beluga, Pinctada fucata (Pearl oyster), Coquille Saint-Jacques, Artemia sp. (Brine shrimp)
    Hôte
    • 73
    • 27
    • 7
    • 2
    Souris
    Clonalité
    • 70
    • 38
    Monoclonal
    Conjugué
    • 61
    • 19
    • 11
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Hsc70 est non-conjugé
    Application
    • 95
    • 53
    • 40
    • 32
    • 30
    • 30
    • 17
    • 15
    • 11
    • 11
    • 11
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificité
    Detects 72 and 73 kDa proteins corresponding to the Molecular Mass of inducible HSP and HSC70 on SDS PAGE Immunoblots.
    Attributs du produit
    Synonyms: HSP73, HSPA10, Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8
    Purification
    Affinity Chromatography on Protein G
    Immunogène
    Chicken Hsp70/Hsp90 complex (1)
    Clone
    BB70
    Isotype
    IgG2a
    Top Product
    Discover our top product HSPA8 Anticorps primaire
  • Indications d'application
    Western blot (7): 1 μg/mL was sufficient for detection of hsp70/Hsc70 in 20 g of HeLalysate.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS pH 7.2, 0.09 % Sodium Azide, 50 % Glycerol
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
    Shelf life: one year from despatch.
    Date de péremption
    12 months
  • Vavilis, Delivanoglou, Aggelidou, Stamoula, Mellidis, Kaidoglou, Cheva, Pourzitaki, Chatzimeletiou, Lazou, Albani, Kritis: "Oxygen-Glucose Deprivation (OGD) Modulates the Unfolded Protein Response (UPR) and Inflicts Autophagy in a PC12 Hypoxia Cell Line Model." dans: Cellular and molecular neurobiology, Vol. 36, Issue 5, pp. 701-12, (2016) (PubMed).

    Stamoula, Vavilis, Aggelidou, Kaidoglou, Cheva, Mellidis, Lazou, Haitoglou, Albani, Kritis: "Low Dose Administration of Glutamate Triggers a Non-Apoptotic, Autophagic Response in PC12 Cells." dans: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 37, Issue 5, pp. 1750-8, (2015) (PubMed).

    Taldone, Kang, Patel, Patel, Patel, Rodina, Patel, Gozman, Maharaj, Clement, Lu, Young, Chiosis et al.: "Heat shock protein 70 inhibitors. 2. 2,5'-thiodipyrimidines, 5-(phenylthio)pyrimidines, 2-(pyridin-3-ylthio)pyrimidines, and 3-(phenylthio)pyridines as reversible binders to an allosteric site on ..." dans: Journal of medicinal chemistry, Vol. 57, Issue 4, pp. 1208-24, (2014) (PubMed).

    Rodina, Taldone, Kang, Patel, Koren, Yan, DaGama Gomes, Yang, Patel, Shrestha, Ochiana, Santarossa, Maharaj, Gozman, Cox, Erdjument-Bromage, Hendrickson, Cerchietti, Melnick, Guzman, Chiosis: "Affinity purification probes of potential use to investigate the endogenous Hsp70 interactome in cancer." dans: ACS chemical biology, Vol. 9, Issue 8, pp. 1698-705, (2014) (PubMed).

    Rodina, Patel, Kang, Patel, Baaklini, Wong, Taldone, Yan, Yang, Maharaj, Gozman, Patel, Patel, Chirico, Erdjument-Bromage, Talele, Young, Chiosis: "Identification of an allosteric pocket on human hsp70 reveals a mode of inhibition of this therapeutically important protein." dans: Chemistry & biology, Vol. 20, Issue 12, pp. 1469-80, (2013) (PubMed).

    Guess, Ayoob, Chanley, Manley, Cajaiba, Agrawal, Pengal, Pyle, Becknell, Kopp, Ronkina, Gaestel, Benndorf, Smoyer: "Crucial roles of the protein kinases MK2 and MK3 in a mouse model of glomerulonephritis." dans: PLoS ONE, Vol. 8, Issue 1, pp. e54239, (2013) (PubMed).

  • Antigène
    Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
    Autre désignation
    HSPA8 / HSC70 (HSPA8 Produits)
    Synonymes
    anticorps hsc54, anticorps hsc70, anticorps hsc71, anticorps hsp71, anticorps hsp73, anticorps hspa10, anticorps lap1, anticorps nip71, anticorps HSC54, anticorps HSC70, anticorps HSC71, anticorps HSP71, anticorps HSP73, anticorps HSPA10, anticorps LAP1, anticorps NIP71, anticorps Hsc70, anticorps 2410008N15Rik, anticorps Hsc71, anticorps Hsc73, anticorps Hsp73, anticorps Hspa10, anticorps wu:fb01g06, anticorps wu:fi48b06, anticorps heat shock protein family A (Hsp70) member 8 L homeolog, anticorps heat shock protein family A (Hsp70) member 8, anticorps heat shock 70kDa protein 8, anticorps heat shock protein 8, anticorps hspa8.L, anticorps HSPA8, anticorps Hspa8, anticorps hspa8
    Sujet
    Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.Synonyms: HSP73, HSPA10, Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein
    ID gène
    3312
    UniProt
    P11142
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