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MMP2 anticorps (Matrix Metalloproteinase 2) (Internal Region)

Détail du produit anti-MMP2 anticorps No. ABIN268224, Fournisseur: Connectez-vous pour afficher Nouveau
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Antigène
Synonymes
2-MMP, CG1794, DM2-MMP, Dm2-MMP, Dmel\\CG1794, MMP2, anon-WO0118547.84, dm-2MMP, dmmp2, l(2)02353, mmp2, wu:fa99h12, wu:fk89d01, fgmmp-2, Mmp-2, LOC100135793, Clg4a, GelA, MMP-2, CLG4, CLG4A, MMP-II,  ... voir plus
2-MMP, CG1794, DM2-MMP, Dm2-MMP, Dmel\\CG1794, MMP2, anon-WO0118547.84, dm-2MMP, dmmp2, l(2)02353, mmp2, wu:fa99h12, wu:fk89d01, fgmmp-2, Mmp-2, LOC100135793, Clg4a, GelA, MMP-2, CLG4, CLG4A, MMP-II, MONA, TBE-1 show less
Épitope
Internal Region, AA 400-500
(29), (20), (15), (13), (13), (13), (13), (12), (8), (8), (7), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivité
Poulet, Humain, Souris, Rat (Rattus)
(335), (118), (108), (11), (9), (9), (6), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Hôte
Lapin
(231), (120), (6), (2), (2), (1)
Clonalité
Polyclonal
Conjugué
Cet anticorp MMP2 est non-conjugé
(23), (15), (8), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Application
Transfer Western (WB), Cytométrie de flux (FACS), Immunocytochimie (ICC), Immunofluorescence (IF), Immunohistochimie (IHC), Immunohistochimie (Tissus congelés) (IHC (fro)), Immunohistochimie (Coupes en paraffine) (IHC (p))
(266), (133), (94), (89), (59), (59), (39), (33), (17), (9), (6), (5), (2), (1), (1), (1), (1)
Pubmed 2 références disponible
Fournisseur Connectez-vous pour afficher Nouveau
Numéro du produit fournisseur Connectez-vous pour afficher Nouveau
Quantité 25 μL
Destination France ( )
Disponibilité Envoi sous 6 à 8 jours ouvrables
Immunogène A synthetic peptide made to an internal region of the human MMP2 protein (between residues 400-500)
Specificité This antibody is specific for pro and active forms of MMP2. It does not react with other MMPs tested.
Purification affinity purified
Autre désignation MMP-2 (MMP2 Antibody Extrait)
Sujet Loss of negative growth regulation and high invasive potential are neoplastic traits oftenassociated with abnormal expression of matrix matalloproteinases. MMP-2, thesignaling mechanism for cells to begin migration, triggers the movement when it splitslaminin-5 and exposes and integrin-binding site on this component of epithelialbasement membrane. The changed form of laminin was found in tumors and in tissuesbeing altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity. Alternate Names: anti-72 kDa gelatinase antibody, anti-72kD type IV collagenase antibody, anti-CLG 4Aantibody, anti-CLG4 antibody, anti-CLG4A antibody, anti-Collagenase Type 4 alphaantibody, anti-Gelatinase A antibody, anti-Gelatinase alpha antibody, anti-Gelatinaseneutrophil antibody, anti-Matrix Metalloproteinase 2 antibody.
Gene Symbol: MMP2
ID gène 4313, 17390, 81686
UniProt P08253
Domaine de recherche Extracellular Matrix
Indications d'application This MMP2 antibody is useful for Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluoresence, and Immunohistochemistry on frozen and paraffin-embedded sections. In Western Blot, bands can be seen at approximately 72kDa (proform) and 62kDa (active form). Antigen retrieval is not required for IHC.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunohistochemistry 2-4 µg/mL, Immunohistochemistry-Frozen 2-4 µg/mL, Immunohistochemistry-Paraffin 2-4 µg/mL, Western Blot 1:1500-1:3500
Protocole Immunohistochemistry-Paraffin Protocol Specific for NB200-193: MMP2 AntibodyMaterials1) 1 Phosphate buffered saline (pH 7.6): NaCl 137mmol/L, KCl 2.7mmol/L, Na2HPO4 4.3mmol/L, KH2PO4 1.4 mmol/L2) Citrate buffer, 0.01 M, pH6.0, Sodium Citrate 3g, Citric acid 0.4g3) 3 % Hydrogen peroxide4) Primary antibody5) Blocking serum (normal serum)6) Biotinylated secondary antibody7) DAB staining kitMethods
1. Dewax and hydration of slides using xylene and EtOH:Dry slides for 20 min in a 60 C ovenAdd Xylene, 2 x 10 min100 %, 95 %, 80 %, and 70 % EtOH, 5 min each EtOH concentration Rinse in PBS, 5'2 Antigen retrieval method (only for paraffin slides)1a. High-pressure antigen retrieval procedure (recommended method)Place slides in a glass slide holder (ensure that the slide holder is completely filled with slides, slides without sections if necessary, to ensure even heating. The entire slide holder is immersed in 1000 mL of Citrate buffer (0.01M, pH6.
0) within a pressure cookerOnce steam is produced, and ONLY when steam is visible, from the pressure cooker (usually 15-20 min), the required high-pressure will have been reached, and slides will be incubated for 2 min.Turn off heat, and allow buffer and slides to cool to room temperatureSlides are then rinsed in PBS for 5 minutes
. Add 3 % hydrogen peroxide solution, 10'at RT, then PBS, 3X5'
. Normal blocking serum, 20'at RT
. Incubate with Primary Ab, 4C overnight or 1.5 hours at 37C
. Rinse with PBS, 3 X 5' each rinse
. Add Biotin-conjugated second antibody, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Add Streptavidin-Peroxidase, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Staining with DAB solution, 2-5'under microscope
. Stop the reaction by washing in tap water
. Counterstain in Haematoxylin for 3-5 minutes
. 75 %, 80 %, 95 % and 100 % ethanol, 5x2', xylene 2 x 10'
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock 4 °C
Stockage commentaire 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Immunohistochimie (IHC) image for anti-MMP2 anticorps (Matrix Metalloproteinase 2) (Internal Region) (ABIN268224) Detection of MMP2 in a paraffin-embedded human neurofibroma section. Stained using A...
Immunohistochimie (IHC) image for anti-MMP2 anticorps (Matrix Metalloproteinase 2) (Internal Region) (ABIN268224) Detection of MMP2 in a frozen injured rat nerve tissue section. Stained using ABIN15...
Transfer Western (WB) image for anti-MMP2 anticorps (Matrix Metalloproteinase 2) (Internal Region) (ABIN268224) Detection of MMP2 in a Western blot of recombinant MMP2 and injured rat nerve lysate ...
Produit citée dans: Horak, Lee, Elkahloun et al.: "Nm23-H1 suppresses tumor cell motility by down-regulating the lysophosphatidic acid receptor EDG2." dans: Cancer research, Vol. 67, Issue 15, pp. 7238-46, 2007 (PubMed).

Krekoski, Neubauer, Graham et al.: "Metalloproteinase-dependent predegeneration in vitro enhances axonal regeneration within acellular peripheral nerve grafts." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 23, pp. 10408-15, 2002 (PubMed).

N° du produit ABIN268224
220,00 €
Plus frais de livraison 40,00 € et TVA

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