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MMP2 anticorps (Matrix Metalloproteinase 2) (Internal Region)

Détail du produit anti-MMP2 anticorps No. ABIN268224, Fournisseur: Connectez-vous pour afficher
Antigène
  • 2-MMP
  • CG1794
  • DM2-MMP
  • Dm2-MMP
  • Dmel\\CG1794
  • MMP2
  • anon-WO0118547.84
  • dm-2MMP
  • dmmp2
  • l(2)02353
  • mmp2
  • wu:fa99h12
  • wu:fk89d01
  • fgmmp-2
  • Mmp-2
  • LOC100135793
  • Clg4a
  • GelA
  • MMP-2
  • CLG4
  • CLG4A
  • MMP-II
  • MONA
  • TBE-1
Épitope
Internal Region, AA 400-500
22
18
15
13
13
13
13
12
8
7
6
6
5
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivité
Poulet, Humain, Souris, Rat (Rattus)
307
118
110
11
9
8
6
5
2
1
1
1
1
1
1
1
1
Hôte
Lapin
212
108
6
2
2
1
Clonalité
Polyclonal
Conjugué
Cet anticorp MMP2 est non-conjugé
20
15
8
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
241
127
91
79
54
51
37
33
16
8
6
5
2
1
1
1
1
Fournisseur
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Supplier Product No.
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Immunogène A synthetic peptide made to an internal region of the human MMP2 protein (between residues 400-500)
Specificité This antibody is specific for pro and active forms of MMP2. It does not react with other MMPs tested.
Purification affinity purified
Autre désignation MMP-2 (MMP2 Antibody Extrait)
Sujet Loss of negative growth regulation and high invasive potential are neoplastic traits oftenassociated with abnormal expression of matrix matalloproteinases. MMP-2, thesignaling mechanism for cells to begin migration, triggers the movement when it splitslaminin-5 and exposes and integrin-binding site on this component of epithelialbasement membrane. The changed form of laminin was found in tumors and in tissuesbeing altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity. Alternate Names: anti-72 kDa gelatinase antibody, anti-72kD type IV collagenase antibody, anti-CLG 4Aantibody, anti-CLG4 antibody, anti-CLG4A antibody, anti-Collagenase Type 4 alphaantibody, anti-Gelatinase A antibody, anti-Gelatinase alpha antibody, anti-Gelatinaseneutrophil antibody, anti-Matrix Metalloproteinase 2 antibody.
Gene Symbol: MMP2
ID gène 4313, 17390, 81686
UniProt P08253
Domaine de recherche Extracellular Matrix
Indications d'application This MMP2 antibody is useful for Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluoresence, and Immunohistochemistry on frozen and paraffin-embedded sections. In Western Blot, bands can be seen at approximately 72kDa (proform) and 62kDa (active form). Antigen retrieval is not required for IHC.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunohistochemistry 2-4 µg/mL, Immunohistochemistry-Frozen 2-4 µg/mL, Immunohistochemistry-Paraffin 2-4 µg/mL, Western Blot 1:1500-1:3500
Protocole Immunohistochemistry-Paraffin Protocol Specific for NB200-193: MMP2 AntibodyMaterials1) 1 Phosphate buffered saline (pH 7.6): NaCl 137mmol/L, KCl 2.7mmol/L, Na2HPO4 4.3mmol/L, KH2PO4 1.4 mmol/L2) Citrate buffer, 0.01 M, pH6.0, Sodium Citrate 3g, Citric acid 0.4g3) 3 % Hydrogen peroxide4) Primary antibody5) Blocking serum (normal serum)6) Biotinylated secondary antibody7) DAB staining kitMethods
1. Dewax and hydration of slides using xylene and EtOH:Dry slides for 20 min in a 60 C ovenAdd Xylene, 2 x 10 min100 %, 95 %, 80 %, and 70 % EtOH, 5 min each EtOH concentration Rinse in PBS, 5'2 Antigen retrieval method (only for paraffin slides)1a. High-pressure antigen retrieval procedure (recommended method)Place slides in a glass slide holder (ensure that the slide holder is completely filled with slides, slides without sections if necessary, to ensure even heating. The entire slide holder is immersed in 1000 mL of Citrate buffer (0.01M, pH6.
0) within a pressure cookerOnce steam is produced, and ONLY when steam is visible, from the pressure cooker (usually 15-20 min), the required high-pressure will have been reached, and slides will be incubated for 2 min.Turn off heat, and allow buffer and slides to cool to room temperatureSlides are then rinsed in PBS for 5 minutes
. Add 3 % hydrogen peroxide solution, 10'at RT, then PBS, 3X5'
. Normal blocking serum, 20'at RT
. Incubate with Primary Ab, 4C overnight or 1.5 hours at 37C
. Rinse with PBS, 3 X 5' each rinse
. Add Biotin-conjugated second antibody, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Add Streptavidin-Peroxidase, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Staining with DAB solution, 2-5'under microscope
. Stop the reaction by washing in tap water
. Counterstain in Haematoxylin for 3-5 minutes
. 75 %, 80 %, 95 % and 100 % ethanol, 5x2', xylene 2 x 10'
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock 4 °C
Stockage commentaire 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Immunohistochemistry (IHC) image for anti-MMP2 anticorps (Matrix Metalloproteinase 2) (Internal Region) (ABIN268224) Detection of MMP2 in a paraffin-embedded human neurofibroma section. Stained using A...
Immunohistochemistry (IHC) image for anti-MMP2 anticorps (Matrix Metalloproteinase 2) (Internal Region) (ABIN268224) Detection of MMP2 in a frozen injured rat nerve tissue section. Stained using ABIN15...
Western Blotting (WB) image for anti-MMP2 anticorps (Matrix Metalloproteinase 2) (Internal Region) (ABIN268224) Detection of MMP2 in a Western blot of recombinant MMP2 and injured rat nerve lysate ...
Produit citée dans: Horak, Lee, Elkahloun et al.: "Nm23-H1 suppresses tumor cell motility by down-regulating the lysophosphatidic acid receptor EDG2." dans: Cancer research, Vol. 67, Issue 15, pp. 7238-46, 2007 (PubMed).

Krekoski, Neubauer, Graham et al.: "Metalloproteinase-dependent predegeneration in vitro enhances axonal regeneration within acellular peripheral nerve grafts." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 23, pp. 10408-15, 2002 (PubMed).