MMP2 anticorps (Matrix Metalloproteinase 2) (Internal Region)

Details for Product anti-MMP2 Antibody No. ABIN268224, Fournisseur: Connectez-vous pour afficher
Antigène
  • 2-MMP
  • CG1794
  • DM2-MMP
  • Dm2-MMP
  • Dmel\\CG1794
  • MMP2
  • anon-WO0118547.84
  • dm-2MMP
  • dmmp2
  • l(2)02353
  • mmp2
  • wu:fa99h12
  • wu:fk89d01
  • fgmmp-2
  • Mmp-2
  • LOC100135793
  • Clg4a
  • GelA
  • MMP-2
  • CLG4
  • CLG4A
  • MMP-II
  • MONA
  • TBE-1
  • matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
  • Matrix metalloproteinase 2
  • matrix metalloproteinase 2
  • matrix metalloproteinase-2
  • matrix metallopeptidase 2
  • MMP2
  • Mmp2
  • mmp2
  • mmp-2
  • Mmp-2
  • LOC100135793
Épitope
Internal Region, AA 400-500
20
18
17
15
15
15
12
11
10
10
8
8
7
6
6
5
5
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Poulet, Cobaye, Humain, Souris, Rat (Rattus)
409
189
180
32
23
22
15
15
8
6
5
5
5
5
5
4
2
2
2
1
1
1
1
Hôte
Lapin
230
193
4
2
2
1
Clonalité
Polyclonal
Conjugué
Cet anticorp MMP2 est non-conjugé
22
17
13
9
9
8
8
7
6
6
6
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
326
173
115
98
98
87
79
60
41
39
6
5
3
3
1
1
1
Options
Fournisseur
Connectez-vous pour afficher
Supplier Product No.
Connectez-vous pour afficher
Immunogène A synthetic peptide made to an internal region of the human MMP2 protein (between residues 400-500) [UniProt P08253]
Specificité This antibody is specific for pro and active forms of MMP2. It was tested in WB againsst recombinant mouse MMP-2, MMP3, MMP7, MMP9, and MMP16 wherein it did not react with other MMPs.
Purification Immunogen affinity purified
Autre désignation MMP-2 (MMP2 Antibody Extrait)
Sujet Gene Symbol: MMP2
Poids moléculaire Theoretical MW: 74 kDa
ID gène 4313
UniProt P08253
Domaine de recherche Extracellular Matrix
Pathways Activation of Innate immune Response
Indications d'application Western Blot 1:1500-1:3500, Immunohistochemistry 2-4 μg/mL, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunohistochemistry-Paraffin 2-4 μg/mL, Immunohistochemistry-Frozen 2-4 μg/mLThis MMP2 antibody is useful for Western Blot, Immunocytochemistry/Immunofluoresence, and Immunohistochemistry on frozen and paraffin-embedded sections. In Western Blot, bands can be seen at approximately 72 kDa (proform) and 62 kDa (active form). Antigen retrieval is not required for IHC. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Commentaires

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocole Immunohistochemistry-Paraffin Protocol Specific for NB200-193: MMP2 AntibodyMaterials1) 1 Phosphate buffered saline (pH 7.6): NaCl 137mmol/L, KCl 2.7mmol/L, Na2HPO4 4.3mmol/L, KH2PO4 1.4 mmol/L2) Citrate buffer, 0.01 M, pH6.0, Sodium Citrate 3g, Citric acid 0.4g3) 3 % Hydrogen peroxide4) Primary antibody5) Blocking serum (normal serum)6) Biotinylated secondary antibody7) DAB staining kitMethods
1. Dewax and hydration of slides using xylene and EtOH:Dry slides for 20 min in a 60 C ovenAdd Xylene, 2 x 10 min100 %, 95 %, 80 %, and 70 % EtOH, 5 min each EtOH concentration Rinse in PBS, 5'2 Antigen retrieval method (only for paraffin slides)1a. High-pressure antigen retrieval procedure (recommended method)Place slides in a glass slide holder (ensure that the slide holder is completely filled with slides, slides without sections if necessary, to ensure even heating. The entire slide holder is immersed in 1000 mL of Citrate buffer (0.01M, pH6.
0) within a pressure cookerOnce steam is produced, and ONLY when steam is visible, from the pressure cooker (usually 15-20 min), the required high-pressure will have been reached, and slides will be incubated for 2 min.Turn off heat, and allow buffer and slides to cool to room temperatureSlides are then rinsed in PBS for 5 minutes
. Add 3 % hydrogen peroxide solution, 10'at RT, then PBS, 3X5'
. Normal blocking serum, 20'at RT
. Incubate with Primary Ab, 4C overnight or 1.5 hours at 37C
. Rinse with PBS, 3 X 5' each rinse
. Add Biotin-conjugated second antibody, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Add Streptavidin-Peroxidase, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Staining with DAB solution, 2-5'under microscope
. Stop the reaction by washing in tap water
. Counterstain in Haematoxylin for 3-5 minutes
. 75 %, 80 %, 95 % and 100 % ethanol, 5x2', xylene 2 x 10'
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid freeze-thaw cycles
Stock 4 °C,-20 °C
Stockage commentaire Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Matrix Metalloproteinase 2 (MMP2) (AA 400-500), (Internal Region) antibody (ABIN268224) Detection of MMP2 in a paraffin-embedded human neurofibroma section. Stained using A...
Immunohistochemistry (IHC) image for anti-Matrix Metalloproteinase 2 (MMP2) (AA 400-500), (Internal Region) antibody (ABIN268224) Detection of MMP2 in a frozen injured rat nerve tissue section. Stained using ABIN15...
Western Blotting (WB) image for anti-Matrix Metalloproteinase 2 (MMP2) (AA 400-500), (Internal Region) antibody (ABIN268224) Detection of MMP2 in a Western blot of recombinant MMP2 and injured rat nerve lysate ...
Western Blotting (WB) image for anti-Matrix Metalloproteinase 2 (MMP2) (AA 400-500), (Internal Region) antibody (ABIN268224) Western Blot: MMP-2 Antibody [ABIN152329] - WB analysis of recombinant pro and active...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Matrix Metalloproteinase 2 (MMP2) (AA 400-500), (Internal Region) antibody (ABIN268224) Immunohistochemistry-Paraffin: MMP-2 Antibody [ABIN152329] - IHC analysis of a formal...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Matrix Metalloproteinase 2 (MMP2) (AA 400-500), (Internal Region) antibody (ABIN268224) Immunohistochemistry-Paraffin: MMP-2 Antibody [ABIN152329] - IHC analysis of a formal...
Immunofluorescence (IF) image for anti-Matrix Metalloproteinase 2 (MMP2) (AA 400-500), (Internal Region) antibody (ABIN268224) Immunocytochemistry/Immunofluorescence: MMP-2 Antibody [ABIN152329] - analysis of MMP...
Produit citée dans: Chuanyu, Yuqing, Chong, Guowei, Xiaojun: "Periostin promotes migration and invasion of renal cell carcinoma through the integrin/focal adhesion kinase/c-Jun N-terminal kinase pathway." dans: Tumour biology, Vol. 39, Issue 4, pp. 1010428317694549, 2017 (PubMed). Méthode utilisée: Western Blotting (WB) (Échantillon (espèces): Human).

Achard, Sanchez, Tassistro, Verdier, Alessi, Grino: "Immediate Postnatal Overfeeding in Rats Programs Aortic Wall Structure Alterations and Metalloproteinases Dysregulation in Adulthood." dans: American journal of hypertension, Vol. 29, Issue 6, pp. 719-26, 2016 (PubMed). (Échantillon (espèces): Rat (Rattus)). Détails: Immunohistochemistry (Paraffin-embedded Sections)

Deng, Tian, Zhou, Zhang, Meng: "Effect of Bilberry Extract on Development of Form-Deprivation Myopia in the Guinea Pig." dans: Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, Vol. 32, Issue 4, pp. 196-202, 2016 (PubMed). (Échantillon (espèces): Guinea Pig). Détails: Western Blotting

Hammers, Sleeper, Forbes, Shima, Walter, Sweeney: "Tadalafil Treatment Delays the Onset of Cardiomyopathy in Dystrophin-Deficient Hearts." dans: Journal of the American Heart Association, Vol. 5, Issue 8, 2016 (PubMed). Détails: Western Blotting

Kasetti, Phan, Millar, Zode: "Expression of Mutant Myocilin Induces Abnormal Intracellular Accumulation of Selected Extracellular Matrix Proteins in the Trabecular Meshwork." dans: Investigative ophthalmology & visual science, Vol. 57, Issue 14, pp. 6058-6069, 2016 (PubMed).

Peetermans, Vanassche, Liesenborghs, Claes, Vande Velde, Kwiecinksi, Jin, De Geest, Hoylaerts, Lijnen, Verhamme: "Plasminogen activation by staphylokinase enhances local spreading of S. aureus in skin infections." dans: BMC microbiology, Vol. 14, pp. 310, 2015 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting

Wang, Xu, Ding, Kim, Zhang, Hai, Yan: "Loss of ATF3 promotes Akt activation and prostate cancer development in a Pten knockout mouse model." dans: Oncogene, Vol. 34, Issue 38, pp. 4975-84, 2015 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Immunohistochemistry (Paraffin-embedded Sections)

Nguyen, Sievers, Motion, Kivimäe, Fang, Lee: "Delivery of lipid micelles into infarcted myocardium using a lipid-linked matrix metalloproteinase targeting peptide." dans: Molecular pharmaceutics, Vol. 12, Issue 4, pp. 1150-7, 2015 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Immunohistochemistry (Frozen Sections)

Li, Sheu, Ma, Pan, Sheu, Wu: "Anti-Restenotic Roles of Dihydroaustrasulfone Alcohol Involved in Inhibiting PDGF-BB-Stimulated Proliferation and Migration of Vascular Smooth Muscle Cells." dans: Marine drugs, Vol. 13, Issue 5, pp. 3046-60, 2015 (PubMed). (Échantillon (espèces): Rat (Rattus)). Détails: Western Blotting

Kamekura, Nava, Feng, Quiros, Nishio, Weber, Parkos, Nusrat: "Inflammation-induced desmoglein-2 ectodomain shedding compromises the mucosal barrier." dans: Molecular biology of the cell, Vol. 26, Issue 18, pp. 3165-77, 2015 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting

Kendziorski, Belcher: "Strain-specific induction of endometrial periglandular fibrosis in mice exposed during adulthood to the endocrine disrupting chemical bisphenol A." dans: Reproductive toxicology (Elmsford, N.Y.), Vol. 58, pp. 119-30, 2015 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Tateossian, Morse, Simon, Dean, Brown: "Interactions between the otitis media gene, Fbxo11, and p53 in the mouse embryonic lung." dans: Disease models & mechanisms, Vol. 8, Issue 12, pp. 1531-42, 2015 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting

Mátyás, Németh, Oláh, Hidi, Birtalan, Kellermayer, Ruppert, Korkmaz-Icöz, Kökény, Horváth, Szabó, Merkely, Radovits: "The soluble guanylate cyclase activator cinaciguat prevents cardiac dysfunction in a rat model of type-1 diabetes mellitus." dans: Cardiovascular diabetology, Vol. 14, pp. 145, 2015 (PubMed). (Échantillon (espèces): Rat (Rattus)). Détails: Western Blotting

Schlage, Egli, Nanni, Wang, Kizhakkedathu, Apte, auf dem Keller: "Time-resolved analysis of the matrix metalloproteinase 10 substrate degradome." dans: Molecular & cellular proteomics : MCP, Vol. 13, Issue 2, pp. 580-93, 2014 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Western Blotting

Han, Baek, Kim, Lee, Ryu, Oh: "Development of an aptamer-conjugated fluorescent nanoprobe for MMP2." dans: Nanoscale research letters, Vol. 9, Issue 1, pp. 104, 2014 (PubMed). Détails: Western Blotting,Immunohistochemistry (Frozen Sections)

Li, Pan, Sheu, Wu, Ma, Wu: "Deep sea water prevents balloon angioplasty-induced hyperplasia through MMP-2: an in vitro and in vivo study." dans: PLoS ONE, Vol. 9, Issue 5, pp. e96927, 2014 (PubMed). (Échantillon (espèces): Rat (Rattus)). Détails: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Lamarca, Gella, Martiañez, Segura, Figueiro-Silva, Grijota-Martinez, Trullas, Casals: "Uridine 5'-triphosphate promotes in vitro Schwannoma cell migration through matrix metalloproteinase-2 activation." dans: PLoS ONE, Vol. 9, Issue 6, pp. e98998, 2014 (PubMed). (Échantillon (espèces): Rat (Rattus)). Détails: Immunocytochemistry,Western Blotting,Immunofluorescence

Hadler-Olsen, Solli, Hafstad, Winberg, Uhlin-Hansen: "Intracellular MMP-2 activity in skeletal muscle is associated with type II fibers." dans: Journal of cellular physiology, Vol. 230, Issue 1, pp. 160-9, 2014 (PubMed). Détails: Immunohistochemistry (Paraffin-embedded Sections),Immunoelectron Microscopy

Solli, Fadnes, Winberg, Uhlin-Hansen, Hadler-Olsen: "Tissue- and cell-specific co-localization of intracellular gelatinolytic activity and matrix metalloproteinase 2." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 61, Issue 6, pp. 444-61, 2013 (PubMed). (Échantillon (espèces): Mouse (Murine)). Détails: Immunohistochemistry (Paraffin-embedded Sections)

Hafstad, Lund, Hadler-Olsen, Höper, Larsen, Aasum: "High- and moderate-intensity training normalizes ventricular function and mechanoenergetics in mice with diet-induced obesity." dans: Diabetes, Vol. 62, Issue 7, pp. 2287-94, 2013 (PubMed).

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