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Cardiac Troponin T2 anticorps

cTnT Reactivité: Humain, Souris, Rat, Porc, Lapin, Chien, Poulet, Cobaye WB, ICS, IHC (f) Hôte: Souris Monoclonal 13 unconjugated
N° du produit ABIN2688865
  • Antigène Voir toutes Cardiac Troponin T2 (cTnT) Anticorps
    Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))
    Reactivité
    • 92
    • 38
    • 31
    • 6
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Porc, Lapin, Chien, Poulet, Cobaye
    Hôte
    • 64
    • 39
    • 2
    • 1
    Souris
    Clonalité
    • 62
    • 43
    Monoclonal
    Conjugué
    • 55
    • 13
    • 9
    • 7
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Cet anticorp Cardiac Troponin T2 est non-conjugé
    Application
    • 58
    • 51
    • 29
    • 11
    • 11
    • 10
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Intracellular Staining (ICS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Marque
    BD Pharmingen™
    Attributs du produit
    The 13-11 monoclonal antibody specifically recognizes Troponin T type 2 (cardiac), encoded by the gene TNNT2. Troponin T is the tropomyosin-binding subunit of the troponin complex, which also encompasses troponin C and troponin I. This complex regulates muscle contraction in skeletal and cardiac muscle in response to alterations in calcium levels. Troponin T type 2 is solely found in the heart, and genetic alterations in the TNNT2 gene are associated to a series of heart disorders in humans, including hypertrophic cardiomyopathy, dilated cardiomyopathy and left ventricular noncompaction. Cardiac Troponin T can be used as a marker for the identification of cardiomyocytes derived from pluripotent stem cells. LEFT PANEL: Western blot analysis of Cardiac Troponin T expression in human and mouse tissues. Human heart lysate was obtained from Abcam (Cat .No. ab2943, lane 1). C2C12 mouse myoblasts (ATCC CRL-1772) were cultured in DMEM containing 10 % FBS (lane 2) or 0.5 % FBS for 14 days for induction of cell differentiation (lane 3). Lysates were prepared, electrophoresed (SDS-PAGE) and transferred to PVDF membranes. The membranes were labeled with 1 μg/mL of Purified Mouse Anti-Cardiac Troponin T. Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat. No. 554002). RIGHT PANEL: Immunohistochemical staining of Cardiac Troponin T in human heart. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No.550878, left) or Purified Mouse Anti-Cardiac Troponin T (right). A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification: 40x. Flow cytometric analysis of Cardiac Troponin T in human (left panel) and mouse (right panel) cardiomyocytes differentiated in vitro. Human embryonic stem cell-derived cardiomyocytes (Evans lab, UCSD) were disassociated and fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm/Wash buffer I (Cat. No. 557885). The C2C12 mouse myoblast cell line (ATCC CRL-1772) was cultured for 5 days in low-serum conditions for induction of cell differentiation, fixed with BD Cytofix™ Fixation Buffer, and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121, dashed line) or Purified Mouse Anti-Cardiac Troponin T (solid line). The second-step reagents were FITC Goat Anti-Mouse Ig (Cat. No. 554001, left) and PE Goat Anti-Mouse Ig (Cat. No. 550589, right). Events with the forward and side light-scatter characteristics of intact cells were gated. Flow cytometry was performed on a BD LSR Fortessa™ (left) or a BD FACSCanto™ II (right) flow cytometry system.

    BD Pharmingen™ Purified Mouse Anti-Cardiac Troponin T - Purified - Clone 13-11 - Isotype Mouse IgG1, κ - Reactivity Ms, Hu, Chick, Dog, G Pig, Pig, Rab, Rat - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Rabbit cardiac troponin T Protein
    Clone
    13
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product cTnT Anticorps primaire
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Jáchymová, Muravská, Paleček, Kuchynka, Řeháková, Magage, Kr��l, Zima, Horký, Linhart: "Genetic variation screening of TNNT2 gene in a cohort of patients with hypertrophic and dilated cardiomyopathy." dans: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 61, Issue 2, pp. 169-75, (2012) (PubMed).

    Lian, Hsiao, Wilson, Zhu, Hazeltine, Azarin, Raval, Zhang, Kamp, Palecek: "Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 27, pp. E1848-57, (2012) (PubMed).

    Uosaki, Fukushima, Takeuchi, Matsuoka, Nakatsuji, Yamanaka, Yamashita: "Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression." dans: PLoS ONE, Vol. 6, Issue 8, pp. e23657, (2011) (PubMed).

    Zhang, Wilson, Soerens, Koonce, Yu, Palecek, Thomson, Kamp: "Functional cardiomyocytes derived from human induced pluripotent stem cells." dans: Circulation research, Vol. 104, Issue 4, pp. e30-41, (2009) (PubMed).

    Mauritz, Schwanke, Reppel, Neef, Katsirntaki, Maier, Nguemo, Menke, Haustein, Hescheler, Hasenfuss, Martin: "Generation of functional murine cardiac myocytes from induced pluripotent stem cells." dans: Circulation, Vol. 118, Issue 5, pp. 507-17, (2008) (PubMed).

    Thierfelder, Watkins, MacRae, Lamas, McKenna, Vosberg, Seidman, Seidman: "Alpha-tropomyosin and cardiac troponin T mutations cause familial hypertrophic cardiomyopathy: a disease of the sarcomere." dans: Cell, Vol. 77, Issue 5, pp. 701-12, (1994) (PubMed).

    Malouf, McMahon, Oakeley, Anderson: "A cardiac troponin T epitope conserved across phyla." dans: The Journal of biological chemistry, Vol. 267, Issue 13, pp. 9269-74, (1992) (PubMed).

  • Antigène
    Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))
    Autre désignation
    Cardiac Troponin T (cTnT Produits)
    Synonymes
    anticorps CMH2, anticorps CMPD2, anticorps LVNC6, anticorps RCM3, anticorps TnTC, anticorps cTnT, anticorps Tnt, anticorps CTTG, anticorps Ctt, anticorps RATCTTG, anticorps Tnnt3, anticorps tnnt2, anticorps CT3, anticorps si:ch211-136g2.1, anticorps troponin T2, cardiac type, anticorps troponin T2, cardiac, anticorps troponin T type 2a (cardiac), anticorps troponin T2, cardiac type L homeolog, anticorps troponin T2e, cardiac, anticorps TNNT2, anticorps Tnnt2, anticorps tnnt2a, anticorps tnnt2.L, anticorps tnnt2e
    Sujet
    Synonyms: cTnT, Cardiac Muscle Troponin T, Troponin T type 2, TNNT2
    Poids moléculaire
    35-40 kDa
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