FCER2 anticorps (Biotin)

N° du produit ABIN2689106

Détail du produit anti-FCER2 anticorps (Biotin)

Antigène: FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp FCER2 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The B3B4 antibody reacts with CD23, the low affinity IgE Fc receptor (FcεRII) expressed on mature resting conventional B lymphocytes, but not on B-1 cells (CD5+ B cells) or T lymphocytes. It does not react with high-affinity IgE receptors, as demonstrated on mouse mast cell lines. The regulation of CD23 surface expression on activated B cells appears to be complex, depending upon the mode of activation and the presence of cytokines. IgE synthesis is negatively regulated by CD23, and CD23 expression is upregulated on splenocytes in the presence of IgE. CD23 is also upregulated on follicular dendritic cells in the lymph nodes of immunized mice, and a subset of splenic dendritic cells expresses CD23. The B3B4 antibody abrogates antigen-specific IgE-dependent modulation of immune responses in normal mice. This monoclonal antibody also blocks IgE binding and eosinophil infiltration in the lung of immunized mice. Different in vivo results have been obtained when using the intact B3B4 antibody or the F(ab')2 fragments. B3B4 mAb does not cross-react with rat or human IgE Fc Receptor. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD23 - Biotin - Clone B3B4 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: FcepsilonR isolated from the mouse B hybridoma line O1.2B2

Clone: B3B4

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-FCER2 (Biotin) (ABIN2689106)

Dasic, Juillard, Graber, Herren, Angell, Knowles, Bonnefoy, Kosco-Vilbois, Chvatchko: "Critical role of CD23 in allergen-induced bronchoconstriction in a murine model of allergic asthma." dans: European journal of immunology, Vol. 29, Issue 9, pp. 2957-67, (1999) (PubMed).

Kisselgof, Oettgen: "The expression of murine B cell CD23, in vivo, is regulated by its ligand, IgE." dans: International immunology, Vol. 10, Issue 9, pp. 1377-84, (1998) (PubMed).

Oshiba, Hamelmann, Haczku, Takeda, Conrad, Kikutani, Gelfand: "Modulation of antigen-induced B and T cell responses by antigen-specific IgE antibodies." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 8, pp. 4056-63, (1997) (PubMed).

Pulendran, Lingappa, Kennedy, Smith, Teepe, Rudensky, Maliszewski, Maraskovsky: "Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2222-31, (1997) (PubMed).

Coyle, Wagner, Bertrand, Tsuyuki, Bews, Heusser: "Central role of immunoglobulin (Ig) E in the induction of lung eosinophil infiltration and T helper 2 cell cytokine production: inhibition by a non-anaphylactogenic anti-IgE antibody." dans: The Journal of experimental medicine, Vol. 183, Issue 4, pp. 1303-10, (1996) (PubMed).

Stief, Texido, Sansig, Eibel, Le Gros, van der Putten: "Mice deficient in CD23 reveal its modulatory role in IgE production but no role in T and B cell development." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3378-90, (1994) (PubMed).

Yu, Kosco-Vilbois, Richards, Köhler, Lamers: "Negative feedback regulation of IgE synthesis by murine CD23." dans: Nature, Vol. 369, Issue 6483, pp. 753-6, (1994) (PubMed).

Maeda, Burton, Padgett, Conrad, Huff, Masuda, Szakal, Tew: "Murine follicular dendritic cells and low affinity Fc receptors for IgE (Fc epsilon RII)." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 8, pp. 2340-7, (1992) (PubMed).

Rabin, Cong, Wortis: "Loss of CD23 is a consequence of B-cell activation. Implications for the analysis of B-cell lineages." dans: Annals of the New York Academy of Sciences, Vol. 651, pp. 130-42, (1992) (PubMed).

Waldschmidt, Snapp, Foy, Tygrett, Carpenter: "B-cell subsets defined by the Fc epsilon R." dans: Annals of the New York Academy of Sciences, Vol. 651, pp. 84-98, (1992) (PubMed).

Waldschmidt, Conrad, Lynch: "Expression of B cell surface receptors. II. IL-4 can accelerate the developmental expression of the murine B cell IgE Fc receptor." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 9, pp. 2820-7, (1989) (PubMed).

Waldschmidt, Conrad, Lynch: "The expression of B cell surface receptors. I. The ontogeny and distribution of the murine B cell IgE Fc receptor." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 7, pp. 2148-54, (1988) (PubMed).

Conrad, Waldschmidt, Lee, Rao, Keegan, Noelle, Lynch, Kehry: "Effect of B cell stimulatory factor-1 (interleukin 4) on Fc epsilon and Fc gamma receptor expression on murine B lymphocytes and B cell lines." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 7, pp. 2290-6, (1987) (PubMed).

Rao, Lee, Conrad: "Characterization of a monoclonal antibody directed against the murine B lymphocyte receptor for IgE." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 6, pp. 1845-51, (1987) (PubMed).

Détails sur FCER2

Antigène: FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))

Autre désignation: CD23 (FCER2 Produits)

Synonymes: anticorps BLAST-2, anticorps CD23, anticorps CD23A, anticorps CLEC4J, anticorps FCE2, anticorps IGEBF, anticorps Fcer2a, anticorps Fc fragment of IgE receptor II, anticorps FCER2, anticorps Fcer2

Sujet: Synonyms: FcεRII

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process