The M-L233 antibody reacts with the low affinity receptor for human IgE, FcεRII/CD23. CD23 is a type II membrane protein which can be expressed by B cells, monocytes, macrophages, eosinophils, platelets, and dendritic cells. CD23 can mediate IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils. Soluble CD23 (sCD23) can be released by CD23+ cells as a result of proteolytic cleavage of membrane CD23. Larger fragments of sCD23 (e.g., 37-25 kDa) retain their IgE-binding capacity whereas smaller fragments (i.e., ≤ 12 kDa) do not. Soluble CD23 may have immunoregulatory effects on the growth and differentiation of B cells and other cell types. Profile of peripheral blood lymphocytes analyzed on a FACScan
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Rieber, Rank, Köhler, Krauss: "Membrane expression of Fc epsilon RII/CD23 and release of soluble CD23 by follicular dendritic cells." dans: Advances in experimental medicine and biology, Vol. 329, pp. 393-8, (1993) (PubMed).
Conrad: "Fc epsilon RII/CD23: the low affinity receptor for IgE." dans: Annual review of immunology, Vol. 8, pp. 623-45, (1990) (PubMed).
Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).
Antigène
FCER2
(Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))