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CD69 anticorps (Biotin)

CD69 Reactivité: Souris FACS Hôte: Hamster arménien Monoclonal H1-2F3 Biotin
N° du produit ABIN2689380
  • Antigène Voir toutes CD69 Anticorps
    CD69
    Reactivité
    • 103
    • 64
    • 13
    • 4
    • 4
    • 4
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    • 1
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    Souris
    Hôte
    • 59
    • 50
    • 29
    • 11
    Hamster arménien
    Clonalité
    • 98
    • 51
    Monoclonal
    Conjugué
    • 40
    • 17
    • 15
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    • 9
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    • 5
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    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CD69 est conjugé à/à la Biotin
    Application
    • 119
    • 52
    • 26
    • 21
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    Flow Cytometry (FACS)
    Marque
    BD Pharmingen™
    Attributs du produit
    The H1.2F3 antibody reacts with CD69 (Very Early Activation antigen), an 85 kDa disulfide-linked homodimer of differentially glycosylated subunits. CD69 is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. Its expression is rapidly induced upon activation of lymphocytes (T, B, NK, and NK-T cells), neutrophils, and macrophages. CD69 is expressed also on thymocytes that are undergoing positive selection, its role in that process is unclear. H1.2F3 mAb augments PMA-induced T-cell stimulation and IFN-γ-induced macrophage stimulation. IL-2-activated NK cells express CD69, and H1.2F3 mAb induces redirected lysis of FcR-bearing target cells by NK cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

    BD Pharmingen™ Biotin Hamster Anti-Mouse CD69 - Biotin - Clone H1.2F3 - Isotype Armenian Hamster IgG1, λ3 - Reactivity Ms - 0.5 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Mouse Dendritic Epidermal T Cell Line Y245
    Clone
    H1-2F3
    Isotype
    IgG1 lambda
    Top Product
    Discover our top product CD69 Anticorps primaire
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Lauzurica, Sancho, Torres, Albella, Marazuela, Merino, Bueren, Martínez-A, Sánchez-Madrid: "Phenotypic and functional characteristics of hematopoietic cell lineages in CD69-deficient mice." dans: Blood, Vol. 95, Issue 7, pp. 2312-20, (2000) (PubMed).

    Keefe, Dave, Allman, Wiest, Kappes: "Regulation of lineage commitment distinct from positive selection." dans: Science (New York, N.Y.), Vol. 286, Issue 5442, pp. 1149-53, (1999) (PubMed).

    Gabor, Godfrey, Scollay: "Recent thymic emigrants are distinct from most medullary thymocytes." dans: European journal of immunology, Vol. 27, Issue 8, pp. 2010-5, (1997) (PubMed).

    Marzio, Jirillo, Ransijn, Mauël, Corradin: "Expression and function of the early activation antigen CD69 in murine macrophages." dans: Journal of leukocyte biology, Vol. 62, Issue 3, pp. 349-55, (1997) (PubMed).

    Merkenschlager, Graf, Lovatt, Bommhardt, Zamoyska, Fisher: "How many thymocytes audition for selection?" dans: The Journal of experimental medicine, Vol. 186, Issue 7, pp. 1149-58, (1997) (PubMed).

    Punt, Suzuki, Granger, Sharrow, Singer: "Lineage commitment in the thymus: only the most differentiated (TCRhibcl-2hi) subset of CD4+CD8+ thymocytes has selectively terminated CD4 or CD8 synthesis." dans: The Journal of experimental medicine, Vol. 184, Issue 6, pp. 2091-9, (1997) (PubMed).

    Wilkinson, Anderson, Owen, Jenkinson: "Positive selection of thymocytes involves sustained interactions with the thymic microenvironment." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 11, pp. 5234-40, (1995) (PubMed).

    Ziegler, Ramsdell, Alderson: "The activation antigen CD69." dans: Stem cells (Dayton, Ohio), Vol. 12, Issue 5, pp. 456-65, (1995) (PubMed).

    Brändle, Müller, Müller, Hengartner, Pircher: "Regulation of RAG-1 and CD69 expression in the thymus during positive and negative selection." dans: European journal of immunology, Vol. 24, Issue 1, pp. 145-51, (1994) (PubMed).

    Ziegler, Levin, Johnson, Copeland, Gilbert, Jenkins, Baker, Sutherland, Feldhaus, Ramsdell: "The mouse CD69 gene. Structure, expression, and mapping to the NK gene complex." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1228-36, (1994) (PubMed).

    Sobel, Yokoyama, Shevach, Eisenberg, Cohen: "Aberrant expression of the very early activation antigen on MRL/Mp-lpr/lpr lymphocytes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 2, pp. 673-82, (1993) (PubMed).

    Bendelac, Matzinger, Seder, Paul, Schwartz: "Activation events during thymic selection." dans: The Journal of experimental medicine, Vol. 175, Issue 3, pp. 731-42, (1992) (PubMed).

    Karlhofer, Yokoyama: "Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. IL-2-activated NK cells possess additional specific stimulation pathways." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 10, pp. 3662-73, (1991) (PubMed).

    Yokoyama, Maxfield, Shevach: "Very early (VEA) and very late (VLA) activation antigens have distinct functions in T lymphocyte activation." dans: Immunological reviews, Vol. 109, pp. 153-76, (1989) (PubMed).

    Yokoyama, Koning, Kehn, Pereira, Stingl, Coligan, Shevach: "Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 2, pp. 369-76, (1988) (PubMed).

  • Antigène
    CD69
    Autre désignation
    CD69 (CD69 Produits)
    Synonymes
    anticorps 5830438K24Rik, anticorps AI452015, anticorps AIM, anticorps VEA, anticorps BL-AC/P26, anticorps CLEC2C, anticorps EA1, anticorps GP32/28, anticorps MLR-3, anticorps Cd69 molecule, anticorps CD69 antigen, anticorps CD69 molecule, anticorps Cd69, anticorps CD69
    Sujet
    Synonyms: Very Early Activation antigen
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