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FAS anticorps

FAS Reactivité: Souris FACS, IP, CyTox Hôte: Hamster arménien Monoclonal Jo2 unconjugated
N° du produit ABIN2689461
  • Antigène Voir toutes FAS Anticorps
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Reactivité
    • 227
    • 68
    • 65
    • 19
    • 9
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Souris
    Hôte
    • 159
    • 102
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Hamster arménien
    Clonalité
    • 156
    • 115
    • 1
    Monoclonal
    Conjugué
    • 130
    • 29
    • 25
    • 12
    • 11
    • 7
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp FAS est non-conjugé
    Application
    • 167
    • 130
    • 75
    • 68
    • 39
    • 36
    • 34
    • 26
    • 26
    • 20
    • 19
    • 12
    • 8
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox)
    Marque
    BD Pharmingen™
    Attributs du produit
    Fas antigen, CD95, is a 45 kDa cell-surface protein which can mediate apoptosis. It belongs to the TNF (tumor necrosis factor)/NGF receptor family. Expression of Fas has been described in the thymus, liver, heart, lung and ovary. Fas plays an important role in the apoptotic process that takes place during development. Monoclonal antibodies recognizing Fas such as Jo2 have cytolytic activity on cells expressing Fas. The cell death stimulated by Fas antibodies is characteristic of apoptosis and suggests that the lethal effects are a result of interaction of antibody with a functional Fas antigen as opposed to complement-mediated lysis. The Jo2 antibody recognizes mouse Fas. The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis. Intraperitoneal injections of Jo2 mAb have been shown to kill mice and induce apoptotic hepatocyte death. Jo2 mAb immunoprecipitates mouse Fas as a 45 kDa band from W4 cells. W4 cells are WR19L mouse lymphoma cells transformed with mouse Fas. The difference between the observed MW of Fas and that deduced from its amino acid sequence (Mr 34,971) may be due to glycosylation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of Fas on mouse thymocytes analyzed by flow cytometry. Thymocytes from a C57BL/6 mouse were incubated with either Jo2, followed by a FITC-conjugated mouse anti-hamster second step (filled histogram, or with second step alone (open histogram).

    BD Pharmingen™ Purified Hamster Anti-Mouse CD95 - Purified - Clone Jo2 - Isotype Armenian Hamster IgG2, λ2 - Reactivity Ms - 0.5 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    WR19L mouse lymphoma cells transformed with recombinant mouse Fas
    Clone
    Jo2
    Isotype
    IgG2 lambda
    Top Product
    Discover our top product FAS Anticorps primaire
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Ogasawara, Suda, Nagata: "Selective apoptosis of CD4+CD8+ thymocytes by the anti-Fas antibody." dans: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 485-91, (1995) (PubMed).

    Yang, Merćep, Ware, Ashwell: "Fas and activation-induced Fas ligand mediate apoptosis of T cell hybridomas: inhibition of Fas ligand expression by retinoic acid and glucocorticoids." dans: The Journal of experimental medicine, Vol. 181, Issue 5, pp. 1673-82, (1995) (PubMed).

    Hiromatsu, Aoki, Makino, Matsumoto, Mizuochi, Gotoh, Nomoto, Ogasawara, Nagata, Yoshikai: "Increased Fas antigen expression in murine retrovirus-induced immunodeficiency syndrome, MAIDS." dans: European journal of immunology, Vol. 24, Issue 10, pp. 2446-51, (1994) (PubMed).

    Kägi, Vignaux, Ledermann, Bürki, Depraetere, Nagata, Hengartner, Golstein: "Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity." dans: Science (New York, N.Y.), Vol. 265, Issue 5171, pp. 528-30, (1994) (PubMed).

    Ogasawara, Watanabe-Fukunaga, Adachi, Matsuzawa, Kasugai, Kitamura, Itoh, Suda, Nagata: "Lethal effect of the anti-Fas antibody in mice." dans: Nature, Vol. 364, Issue 6440, pp. 806-9, (1993) (PubMed).

  • Antigène
    FAS (TNF Receptor Superfamily, Member 6 (FAS))
    Autre désignation
    CD95 (FAS Produits)
    Synonymes
    anticorps ALPS1A, anticorps APO-1, anticorps APT1, anticorps CD95, anticorps FAS1, anticorps FASTM, anticorps TNFRSF6, anticorps AI196731, anticorps APO1, anticorps TNFR6, anticorps Tnfrsf6, anticorps lpr, anticorps Fas cell surface death receptor, anticorps Fas (TNF receptor superfamily member 6), anticorps FAS, anticorps Fas, anticorps fas
    Sujet
    Synonyms: Fas/APO-1
    Poids moléculaire
    45 kDaTarget Molecular Weight: Aqueous buffered solution containing ≤0.09% sodium azide.
    Pathways
    Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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