FAS anticorps (Biotin)

N° du produit ABIN2689463

Détail du produit anti-FAS anticorps (Biotin)

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp FAS est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: Fas antigen, CD95, is a 45 kDa cell-surface protein which can mediate apoptosis. It belongs to the TNF (tumor necrosis factor)/NGF receptor family. Expression of Fas has been described in the thymus, liver, heart, lung and ovary. Fas plays an important role in the apoptotic process that takes place during development. Monoclonal antibodies recognizing Fas such as Jo2 have cytolytic activity on cells expressing Fas. The cell death stimulated by Fas antibodies is characteristic of apoptosis and suggests that the lethal effects are a result of interaction of antibody with a functional Fas antigen as opposed to complement-mediated lysis. The Jo2 antibody recognizes mouse Fas. The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis. Intraperitoneal injections of Jo2 mAb have been shown to kill mice and induce apoptotic hepatocyte death. Jo2 mAb immunoprecipitates mouse Fas as a 45 kDa band from W4 cells. W4 cells are WR19L mouse lymphoma cells transformed with mouse Fas. The difference between the observed MW of Fas and that deduced from its amino acid sequence (Mr 34,971) may be due to glycosylation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Expression of Fas antigen on mouse thymocytes analyzed by flow cytometry. Thymocytes from a BALB/c mouse were incubated with either Jo2 followed by a Streptavidin-PE conjugated second-step (Cat. No. 554061, filled histogram) or with only second step (open histogram). Jo2 specifically stained more than 90 % of the cells.

BD Pharmingen™ Biotin Hamster Anti-Mouse CD95 - Biotin - Clone Jo2 - Isotype Armenian Hamster IgG2, λ2 - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: WR19L mouse lymphoma cells transformed with recombinant mouse Fas

Clone: Jo2

Isotype: IgG2 lambda

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-FAS (Biotin) (ABIN2689463)

Enari, Hug, Nagata: "Involvement of an ICE-like protease in Fas-mediated apoptosis." dans: Nature, Vol. 375, Issue 6526, pp. 78-81, (1995) (PubMed).

Nagata: "Apoptosis regulated by a death factor and its receptor: Fas ligand and Fas." dans: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 345, Issue 1313, pp. 281-7, (1995) (PubMed).

Nagata: "Fas and Fas ligand: a death factor and its receptor." dans: Advances in immunology, Vol. 57, pp. 129-44, (1995) (PubMed).

Ni, Tomita, Matsuda, Ichihara, Ishimura, Ogasawara, Nagata: "Fas-mediated apoptosis in primary cultured mouse hepatocytes." dans: Experimental cell research, Vol. 215, Issue 2, pp. 332-7, (1995) (PubMed).

Ogasawara, Suda, Nagata: "Selective apoptosis of CD4+CD8+ thymocytes by the anti-Fas antibody." dans: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 485-91, (1995) (PubMed).

Hiromatsu, Aoki, Makino, Matsumoto, Mizuochi, Gotoh, Nomoto, Ogasawara, Nagata, Yoshikai: "Increased Fas antigen expression in murine retrovirus-induced immunodeficiency syndrome, MAIDS." dans: European journal of immunology, Vol. 24, Issue 10, pp. 2446-51, (1994) (PubMed).

Kägi, Vignaux, Ledermann, Bürki, Depraetere, Nagata, Hengartner, Golstein: "Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity." dans: Science (New York, N.Y.), Vol. 265, Issue 5171, pp. 528-30, (1994) (PubMed).

Takahashi, Tanaka, Brannan, Jenkins, Copeland, Suda, Nagata: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." dans: Cell, Vol. 76, Issue 6, pp. 969-76, (1994) (PubMed).

Ogasawara, Watanabe-Fukunaga, Adachi, Matsuzawa, Kasugai, Kitamura, Itoh, Suda, Nagata: "Lethal effect of the anti-Fas antibody in mice." dans: Nature, Vol. 364, Issue 6440, pp. 806-9, (1993) (PubMed).

Détails sur FAS

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Autre désignation: CD95 (FAS Produits)

Synonymes: anticorps ALPS1A, anticorps APO-1, anticorps APT1, anticorps CD95, anticorps FAS1, anticorps FASTM, anticorps TNFRSF6, anticorps AI196731, anticorps APO1, anticorps TNFR6, anticorps Tnfrsf6, anticorps lpr, anticorps Fas cell surface death receptor, anticorps Fas (TNF receptor superfamily member 6), anticorps FAS, anticorps Fas, anticorps fas

Sujet: Synonyms: Fas/APO-1

Pathways: Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity