IL-8 anticorps

N° du produit ABIN2689787

Détail du produit anti-IL-8 anticorps

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-8 est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Blocking Antibody (Inhibition)

Marque: BD Pharmingen™

Attributs du produit: The G265-8 antibody reacts with both the 72 and 77 amino acid forms of human interleukin-8 (IL-8). The immunogen used to produce the G265-8 hybridoma was E. coli-expressed recombinant human IL-8. Expression of IL-8 by stimulated CD14+ human monocytes. Human PBMC were stimulated for 6 hours with LPS (10 ng/mL final concentration) in the presence of GolgiStop™ (2 μM final concentration, Cat. No. 554724). The PBMC were harvested, stained with FITC-mouse anti-human CD14 antibody (FITC-M5E2, Cat. No. 555397), fixed, permeabilized, and subsequently stained with 0.25 μg of PE-mouse anti-human IL-8 antibody (PE-G265-8, Cat. No. 554720) following the BD Pharmingen staining protocol (left panel). The data reflect gating on monocytes, based on forward and side scattered light signals. To demonstrate specificity of staining, the binding of PE-G265-8 was blocked by the preincubation of the conjugated antibody with recombinant human IL-8 (0.25 μg, Cat. No. 554609, center panel), and by preincubation of the fixed/permeabilized cells with unlabelled G265-8 antibody (2.5 μg, Cat. No. 554717, right panel) prior to staining with the PE-G265-8 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking (center panel) and unlabelled antibody (right panel) blocking specificity controls.

BD Pharmingen™ Purified Mouse Anti-Human IL-8 - Purified - Clone G265-8 - Isotype Mouse IgG2b - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Recombinant Human IL-8

Clone: G265

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-IL-8 (ABIN2689787)

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." dans: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Matsushima, Oppenheim: "Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF." dans: Cytokine, Vol. 1, Issue 1, pp. 2-13, (1991) (PubMed).

Détails sur IL-8

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Autre désignation: IL-8 (IL8 Produits)

Synonymes: anticorps CXCL8, anticorps GCP-1, anticorps GCP1, anticorps LECT, anticorps LUCT, anticorps LYNAP, anticorps MDNCF, anticorps MONAP, anticorps NAF, anticorps NAP-1, anticorps NAP1, anticorps IL-8, anticorps AMCF-I, anticorps il8, anticorps IL8, anticorps si:dkey-151b16.2, anticorps cxcli2, anticorps cxcl8, anticorps gcp-1, anticorps il-8, anticorps lynap, anticorps mdncf, anticorps monap, anticorps nap-1, anticorps C-X-C motif chemokine ligand 8, anticorps interleukin 8, anticorps chemokine (C-X-C motif) ligand 8a, anticorps chemokine (C-X-C motif) ligand 1-like, anticorps chemokine (C-X-C motif) ligand 8 L homeolog, anticorps CXCL8, anticorps il8, anticorps cxcl8a, anticorps IL8, anticorps Cxcl8, anticorps LOC422654, anticorps cxcl8.L

Pathways: Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy