MYOD1 anticorps

N° du produit ABIN272324

Détail du produit anti-MYOD1 anticorps

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivité: Humain, Souris, Rat, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MYOD1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Purification: Purified

Immunogène: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.

Clone: 5-2F

Isotype: IgG2a

Information d'application

Indications d'application: ELISA. Western Blot: 1 μg/mL. Immunofluorescence. Electron microscopy. Immunoprecipitation: 2 μg/mg of protein lysate. Immunohistochemistry on Frozen and Paraffin Sections: 2-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preserative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur MYOD1

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Autre désignation: MYOD1 (MYOD1 Produits)

Synonymes: anticorps MYF3, anticorps MYOD, anticorps PUM, anticorps bHLHc1, anticorps MyoD, anticorps XMyoD, anticorps XtmyoD, anticorps mf25, anticorps myf3, anticorps pum, anticorps MUF3, anticorps AI503393, anticorps Myod-1, anticorps etID309723.25, anticorps myod, anticorps wu:fb57a01, anticorps zgc:136744, anticorps MYOD1, anticorps myod1, anticorps mf1, anticorps myod1-a, anticorps myod1-b, anticorps myogenic differentiation 1, anticorps MYOD protein, anticorps myogenic factor MyoD1, anticorps myogenic differentiation 1 S homeolog, anticorps myogenic differentiation 1 L homeolog, anticorps MYOD1, anticorps myod1, anticorps Myod1, anticorps myod, anticorps myod1.S, anticorps myod1.L

Sujet: Recognizes a phosphoprotein of 45 kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.Synonyms: BHLHC1, Class C basic helix-loop-helix protein 1, MYF3, MYOD, Myoblast determination protein 1, Myogenic factor 3

ID gène: 10090

UniProt: P10085

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development