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HYAL1 anticorps (N-Term)

HYAL1 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin WB, IHC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN2784343
  • Antigène Voir toutes HYAL1 Anticorps
    HYAL1 (Hyaluronidase-1 (HYAL1))
    Épitope
    • 15
    • 10
    • 9
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 56
    • 7
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin
    Hôte
    • 54
    • 6
    • 1
    Lapin
    Clonalité
    • 58
    • 3
    Polyclonal
    Conjugué
    • 32
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp HYAL1 est non-conjugé
    Application
    • 42
    • 30
    • 22
    • 13
    • 13
    • 10
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Séquence
    TIFYSSQLGT YPYYTPTGEP VFGGLPQNAS LIAHLARTFQ DILAAIPAPD
    Homologie
    Cow: 86%, Dog: 85%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 92%
    Attributs du produit
    This is a rabbit polyclonal antibody against HYAL1. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogène
    The immunogen is a synthetic peptide directed towards the N terminal region of human HYAL1
    Top Product
    Discover our top product HYAL1 Anticorps primaire
  • Indications d'application
    Optimal working dilutions should be determined experimentally by the investigator.
    Commentaires

    Antigen size: 435 AA

    Restrictions
    For Research Use only
  • Validation #100034 (Immunohistochemistry)
    'Independent Validation' signe
    by
    University of Manitoba, Max Rady College of Medicine Biochemistry and Medical Genetics, Department of Biochemistry and Medical Genetics
    No.
    #100034
    Date
    16.08.2016
    Antigène
    HYAL1 antibody - N-terminal region
    Numéro du lot
    QC23783
    Application validée
    Immunohistochemistry
    Contrôle positif
    Testes of Hyal1 +/+ mouse
    Contrôle négative
    Testes of Hyal1 -/- mouse
    Conclusion
    ABIN2784343 does detect mouse HYAL1 by immunohistochemistry in paraffin sections. The immunohistochemistry is based on increased signal in wild type mouse testes compared to Hyal1-/- mouse testes.
    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN2784343
    Anticorps secondaire
    Goat-anti-rabbit AF568
    Full Protocol
    • Deparafinize and rehydrate:
      • xylene (I & II) – 2x 5min
      • 100% Ethanol (I & II) – 2x 5min
      • 95% Ethanol (I & II) – 2x 3min
      • 70% Ethanol (I) – 3min
      • 50% Ethanol (I) – 3min
      • ddH2O – 3min
    • Antigen retrieval:
      • Immerse slides in citrate buffer (10mM Sodium citrate at pH6.0) preheated to 95°C.
      • Incubate at 95°C for 20min.
      • Cool down at RT for 20min.
      • Wash twice with PBS for 5min.
    • Immunostaining:
      • Block in blocking solution (prepare 3% BSA in TBST) at RT for 60min.
      • Incubate sections with primary Hyaluronidase-1 (HYAL1) (N-Term) antibody ABIN2784343 diluted 1:200 in blocking solution.
      • Incubate at 4°C overnight.
      • Wash slides three times with TBST 5min.
      • Incubate sections with goat-anti-rabbit AF568 secondary antibody conjugate diluted 1:500 in blocking solution.
      • Incubate at RT for 1h.
      • Wash slides in TBST - 1x 5min at RT.
      • Wash three times with H2O for 5min.
      • Incubated in Hoechst solution diluted 1:100.000 for 8-9min.
      • Wash twice with H2O for 5min.
      • Mounted with ProLong Gold (ThermoFisher, P36931) solution.
    Notes
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Antigène
    HYAL1 (Hyaluronidase-1 (HYAL1))
    Autre désignation
    HYAL1 (HYAL1 Produits)
    Synonymes
    anticorps HYAL-1, anticorps LUCA1, anticorps NAT6, anticorps Hya1, anticorps Hyal-1, anticorps hyaluronoglucosaminidase 1, anticorps HYAL1, anticorps Hyal1
    Sujet
    HYAL1 is a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.
    Alias Symbols: HYAL-1, LUCA1, MGC45987, NAT6
    Protein Interaction Partner: UBC, COL2A1,
    Protein Size: 435
    Poids moléculaire
    45 kDa
    ID gène
    3373
    NCBI Accession
    NM_153282, NP_695014
    UniProt
    Q12794
    Pathways
    Glycosaminoglycan Metabolic Process
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