Iba1 anticorps (C-Term)

N° du produit ABIN2857032

Détail du produit anti-Iba1 anticorps

Highlights:

• High quality Iba1 Anticorps primaire for the detection of IBA1.
• Reliable product with high standard validation data

Antigène: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Iba1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Free Floating) (IHC (ff))

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to Iba1 (allograft inflammatory factor 1)
Iba1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.

Isotype: IgG

Informations sur le produit:

À quoi peut servir l'anticorps Iba1 ABIN2857032 ? Cet anticorps polyclonal de lapin anti-Iba1 n'est pas conjugué et convient à la détection de l'antigène Iba1 / AIF1 (Allograft Inflammatory Factor 1) chez la souris, l'homme, le rat. Il est validé pour l'IHC et le Western blotting.

Quelles sont les données de validation disponibles pour cet anticorps Iba1 ? 18 images pour IHC et immunomarquage et autres applications sont disponibles. Le produit est cité dans 13 publications PubMed et bénéficie d'une validation par un client indépendant. Utilisez notre anticorps Iba1 de haute qualité pour détecter de manière fiable Iba1 / AIF1.

Quelle est la fonction de Iba1 / AIF1 ? Iba1 est une protéine de liaison à l'actine qui améliore le brassage de la membrane et l'activation du RAC, augmente l'activité de liaison à l'actine de LCP1, lie le calcium et joue un rôle dans la signalisation du RAC, la phagocytose, l'activation et la fonction des macrophages, la prolifération des cellules musculaires lisses vasculaires et des lymphocytes T, la migration des lymphocytes et l'inflammation vasculaire.

Information d'application

Indications d'application: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: mouse liver , rat liver

Validation: Orthogonal

Restrictions: For Research Use only

Independent Validation (IF)

Validation #101924 (Immunofluorescence)

by: Centre d'Immunologie de Marseille-Luminy

No.: #101924

Date: 19.10.2017

Antigène: AIF1

Numéro du lot: 39476

Application validée: Immunofluorescence

Contrôle positif: PFA fixed tissue section brain of CX3CR1 GFP mouse. Staining with Iba1 (Wako, 019-19741) and Donkey anti-rabbit Fab’2 AlexaFluor 647

Conclusion: Passed. Immunostaining of mouse brain samples with ABIN2857032 shows specifically the expected staining pattern.

Validation Images

Colocalization (C) of the signals in PFA fixed tissue sections of CX3CR1 GFP mouse brain for the immunostaining of with ABIN2857032 (A) and GFP (B).

Protocole

Anticorps primaire: ABIN2857032

Anticorps secondaire: donkey Fab’2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806)

Full Protocol:

• Harvest Brain from 13 weeks old mice in 1x Dulbecco’s phosphate buffered saline (DPBS) (Gibco Life Technologies, 14200-067).
• Fix mouse brain in antigen fix (Diapath, P0014) for 2h at RT.
• Wash tissue in 0.1M pH7.4 phosphate buffer at for 1h at 4°C.
• Dehydrate tissue in 30% sucrose solution ON at 4°C.
• Snap freeze tissue in Tissue Freezing Medium (ElectroMicroscopy Science, 72592-C) at -80°C.
• Cut blocks into 20µm sections using a cryostat (Leica, CM3050 S).
• Transfer sections to a slide.
• Create a hydrophobic barrier on the slide around sections with Dako pen (Dako, S2002, lot 00081640).
• Place slide in a humidified chamber and rehydrate sections in 0.1M TrisHCl pH7.4 for 10min at RT.
• Gently remove buffer by tapping slide.
• Permeabilize tissue in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA, for 20min at RT.
• Incubate sections with primary AIF1 (c-term) (antibodies-online, ABIN2857032, Lot 39476) diluted 1:500 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 5h at RT. Incubate the negative contrls sections with diluted rabbit serum.
• Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
• Incubate sections with secondary donkey Fab’2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806) diluted 1:500 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 and 0.5% BSA ON at RT.
• Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
• Add approximately 10μl of Slowfade® Gold antifade reagent (Life technologies, 536937, lot 1226836) for each section and mount cover slip.
• Image acquisition on a LSM 880 (Zeiss), 20x magnification, 1000 resolution.

Notes: Microglial cells in the brain of the CX3CR1 GFP mouse expressing GFP are known to express Aif-1. Immunostaining of the PFA fixed tissue sections with ABIN2857032 showed the expected co-localization of the GFP and Aif-1 signals.

Stockage

Format: Liquid

Concentration: 0.06 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-Iba1 (ABIN2857032)

Kaehler, Seitter, Sandbichler, Tschugg, Obermair, Stefanova, Koschak: "Assessment of the Retina of Plp-α-Syn Mice as a Model for Studying Synuclein-Dependent Diseases." dans: Investigative ophthalmology & visual science, Vol. 61, Issue 6, pp. 12, (2020) (PubMed).

Wahle, Sofranko, Dekkers, Miller, Heusinkveld, Albrecht, Cassee, Schins: "Evaluation of neurological effects of cerium dioxide nanoparticles doped with different amounts of zirconium following inhalation exposure in mouse models of Alzheimer's and vascular disease." dans: Neurochemistry international, Vol. 138, pp. 104755, (2020) (PubMed).

Li, Zhang, Yang, Blevins, Norris, Zhao, Huang: "C-terminal binding proteins 1 and 2 in traumatic brain injury-induced inflammation and their inhibition as an approach for anti-inflammatory treatment." dans: International journal of biological sciences, Vol. 16, Issue 7, pp. 1107-1120, (2020) (PubMed).

Mondor, Baratin, Lagueyrie, Saro, Henri, Gentek, Suerinck, Kastenmuller, Jiang, Bajénoff: "Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage Niche." dans: Immunity, Vol. 50, Issue 6, pp. 1453-1466.e4, (2019) (PubMed).

Olechnowicz, Weivoda, Lwin, Leung, Gooding, Nador, Javaid, Ramasamy, Rao, Edwards, Edwards: "Multiple myeloma increases nerve growth factor and other pain-related markers through interactions with the bone microenvironment." dans: Scientific reports, Vol. 9, Issue 1, pp. 14189, (2019) (PubMed).

Wahl, Solon-Biet, Wang, Wali, Pulpitel, Clark, Raubenheimer, Senior, Sinclair, Cooney, de Cabo, Cogger, Simpson, Le Couteur: "Comparing the Effects of Low-Protein and High-Carbohydrate Diets and Caloric Restriction on Brain Aging in Mice." dans: Cell reports, Vol. 25, Issue 8, pp. 2234-2243.e6, (2019) (PubMed).

Huang, Lin, Chen, Chen, Chen, Hsu, Wu: "NADPH oxidases as potential pharmacological targets against increased seizure susceptibility after systemic inflammation." dans: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 140, (2019) (PubMed).

Götzl, Brendel, Werner, Parhizkar, Sebastian Monasor, Kleinberger, Colombo, Deussing, Wagner, Winkelmann, Diehl-Schmid, Levin, Fellerer, Reifschneider, Bultmann, Bartenstein, Rominger, Tahirovic et al.: "Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism. ..." dans: EMBO molecular medicine, Vol. 11, Issue 6, (2019) (PubMed).

Lin, Hsuan, Lin, Kuo, Lin, Su, Niu, Chang, Lin: "Human Umbilical Cord Mesenchymal Stem Cells Preserve Adult Newborn Neurons and Reduce Neurological Injury after Cerebral Ischemia by Reducing the Number of Hypertrophic Microglia/Macrophages." dans: Cell transplantation, Vol. 26, Issue 11, pp. 1798-1810, (2018) (PubMed).

Zha, Liu, Zhu, Liu, Lu, Xu, Yu, Liu: "A scFv antibody targeting common oligomeric epitope has potential for treating several amyloidoses." dans: Scientific reports, Vol. 6, pp. 36631, (2018) (PubMed).

Zera, Zastre: "Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes." dans: PLoS ONE, Vol. 12, Issue 10, pp. e0186707, (2017) (PubMed).

Liu, Shi, Huang, Peterson, Wu, Lan, Zhang, He, Woods, Du, Wu, Wang: "Astragaloside IV inhibits microglia activation via glucocorticoid receptor mediated signaling pathway." dans: Scientific reports, Vol. 6, pp. 19137, (2016) (PubMed).

Wolf, Damme, Stroobants, DHooge, Beck, Hermans-Borgmeyer, Lüllmann-Rauch, Dierks, Lübke: "A mouse model for fucosidosis recapitulates storage pathology and neurological features of the milder form of the human disease." dans: Disease models & mechanisms, Vol. 9, Issue 9, pp. 1015-28, (2016) (PubMed).

Détails sur Iba1

Antigène: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Autre désignation: allograft inflammatory factor 1 (IBA1 Produits)

Synonymes: anticorps AIF-1, anticorps IBA1, anticorps IRT-1, anticorps IRT1, anticorps AI607846, anticorps D17H6S50E, anticorps G1, anticorps Iba1, anticorps Bart1, anticorps iba1, anticorps mrf-1, anticorps fa04b11, anticorps wu:fa04b11, anticorps AIF, anticorps AIF1, anticorps aif1, anticorps allograft inflammatory factor 1, anticorps AIF1, anticorps Aif1, anticorps aif1

Sujet: This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 17 kDa

ID gène: 199

UniProt: P55008

Pathways: Smooth Muscle Cell Migration