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Myc Tag anticorps (C-Term) (HRP)

Reactivité: Humain WB Hôte: Souris Monoclonal 9E10 HRP
N° du produit ABIN302064
  • Antigène Tous les produits Myc Tag
    Myc Tag
    Épitope
    • 34
    • 22
    • 12
    • 8
    • 7
    • 3
    • 2
    • 1
    • 1
    C-Term
    Reactivité
    • 39
    • 5
    • 1
    Humain
    Hôte
    • 85
    • 48
    • 10
    • 9
    • 7
    • 7
    • 1
    • 1
    Souris
    Clonalité
    • 96
    • 60
    • 10
    Monoclonal
    Conjugué
    • 84
    • 13
    • 12
    • 8
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Myc Tag est conjugé à/à la HRP
    Application
    • 132
    • 66
    • 49
    • 48
    • 36
    • 33
    • 33
    • 21
    • 17
    • 11
    • 11
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Specificité
    The antibody 9E10 can be used to detect the c-Myc tag.
    Réactivité croisée (Details)
    Human, Recognizes fusion proteins in all species
    Purification
    Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.
    Immunogène
    Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
    Clone
    9E10
    Isotype
    IgG1
  • Indications d'application
    Western blotting: Recommended dilution: 1-2 μg/mL, positive control: c-Myc tagged protein.
    Commentaires

    The purified antibody is conjugated with Horseradish Peroxidase (HRP) of high specific activity and RZ=3.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01 % thimerosal
    Agent conservateur
    Thimerosal (Merthiolate)
    Précaution d'utilisation
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Avoid prolonged exposure to light.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Jelínková, Šafaříková, Vondálová Blanářová, Skender, Hofmanová, Sova, Moyer, Kozubík, Kolář, Ehrmann, Hyršlová Vaculová: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." dans: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, (2014) (PubMed).

    Veracini, Simon, Richard, Schraven, Horejsi, Roche, Benistant: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." dans: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, (2008) (PubMed).

    Wang, Campoli, Ko, Luo, Ferrone: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." dans: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, (2004) (PubMed).

    Fujiwara, Poikonen, Aleman, Valtavaara, Saksela, Mayer: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." dans: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, (2002) (PubMed).

    Baggio, Burgstaller, Hale, Putney, Lane, Lipovsek, Wright, Roberts, Liu, Szostak, Wagner: "Identification of epitope-like consensus motifs using mRNA display." dans: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, (2002) (PubMed).

    Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." dans: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).

    Hilpert, Hansen, Wessner, Küttner, Welfle, Seifert, Höhne: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." dans: Protein engineering, Vol. 14, Issue 10, pp. 803-6, (2001) (PubMed).

    Siegel, Brandner, Hess: "Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC." dans: International journal of oncology, Vol. 13, Issue 6, pp. 1259-62, (1999) (PubMed).

  • Antigène
    Myc Tag
    Autre désignation
    c-Myc tag (Myc Tag Produits)
    Classe de substances
    Tag
    Sujet
    MYC proto-oncogene,The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.,bHLH, MRTL, MYCC
    ID gène
    4609
    UniProt
    P01106
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