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Myc Tag anticorps (C-Term)

Reactivité: Humain WB, IP, IHC (p), FACS Hôte: Souris Monoclonal 9E10 unconjugated
N° du produit ABIN302092
  • Antigène Tous les produits Myc Tag
    Myc Tag
    Épitope
    • 34
    • 22
    • 12
    • 8
    • 7
    • 3
    • 2
    • 1
    • 1
    C-Term
    Reactivité
    • 39
    • 5
    • 1
    Humain
    Hôte
    • 85
    • 48
    • 10
    • 9
    • 7
    • 7
    • 1
    • 1
    Souris
    Clonalité
    • 96
    • 60
    • 10
    Monoclonal
    Conjugué
    • 83
    • 14
    • 12
    • 8
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Myc Tag est non-conjugé
    Application
    • 132
    • 65
    • 49
    • 48
    • 35
    • 33
    • 33
    • 20
    • 17
    • 11
    • 11
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
    Specificité
    The antibody 9E10 can be used to detect the c-Myc tag.
    Réactivité croisée (Details)
    Human, Recognizes fusion proteins in all species
    Purification
    Purified by protein-A affinity chromatography.
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
    Clone
    9E10
    Isotype
    IgG1
  • Indications d'application
    Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/mL, positive tissue: perfused brain sections, liver, spleen.
    Immunoprecipitation: Recommended dilution: 1-5 μg/mL, this antibody is not suitable for immunoprecipitation of native c-Myc protein.
    Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-4 μg/mL.
    Western blotting: Recommended dilution: 0,5-2 μg/mL, positive control: c-Myc tagged protein.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Jelínková, Šafaříková, Vondálová Blanářová, Skender, Hofmanová, Sova, Moyer, Kozubík, Kolář, Ehrmann, Hyršlová Vaculová: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." dans: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, (2014) (PubMed).

    Veracini, Simon, Richard, Schraven, Horejsi, Roche, Benistant: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." dans: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, (2008) (PubMed).

    Wang, Campoli, Ko, Luo, Ferrone: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." dans: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, (2004) (PubMed).

    Fujiwara, Poikonen, Aleman, Valtavaara, Saksela, Mayer: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." dans: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, (2002) (PubMed).

    Baggio, Burgstaller, Hale, Putney, Lane, Lipovsek, Wright, Roberts, Liu, Szostak, Wagner: "Identification of epitope-like consensus motifs using mRNA display." dans: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, (2002) (PubMed).

    Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." dans: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).

    Hilpert, Hansen, Wessner, Küttner, Welfle, Seifert, Höhne: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." dans: Protein engineering, Vol. 14, Issue 10, pp. 803-6, (2001) (PubMed).

    Siegel, Brandner, Hess: "Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC." dans: International journal of oncology, Vol. 13, Issue 6, pp. 1259-62, (1999) (PubMed).

  • Antigène
    Myc Tag
    Autre désignation
    c-Myc tag (Myc Tag Produits)
    Classe de substances
    Tag
    Sujet
    MYC proto-oncogene,The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.,bHLH, MRTL, MYCC
    ID gène
    4609
    UniProt
    P01106
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