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Involucrin anticorps

IVL Reactivité: Humain, Porc, Chien, Gorilla, Owl Monkey IF, FACS, IHC (p) Hôte: Souris Monoclonal SY5 unconjugated
N° du produit ABIN3026652
  • Antigène Voir toutes Involucrin (IVL) Anticorps
    Involucrin (IVL)
    Reactivité
    • 89
    • 24
    • 23
    • 17
    • 17
    • 10
    • 9
    • 8
    • 5
    • 2
    Humain, Porc, Chien, Gorilla, Owl Monkey
    Hôte
    • 59
    • 38
    Souris
    Clonalité
    • 51
    • 46
    Monoclonal
    Conjugué
    • 46
    • 10
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Involucrin est non-conjugé
    Application
    • 35
    • 33
    • 28
    • 26
    • 23
    • 21
    • 19
    • 16
    • 13
    • 13
    • 12
    • 10
    • 6
    • 4
    • 2
    • 1
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Aucune reactivité croisée
    Souris
    Attributs du produit
    This antibody recognizes a protein of 66-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between amino acids 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
    Purification
    Protein G affinity chromatography
    Immunogène
    Purified Involucrin from human keratinocytes was used as the immunogen for this antibody.
    Clone
    SY5
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product IVL Anticorps primaire
  • Indications d'application
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues is enhanced by digestion with trypsin Protease XXV (Sigma) at 1 mg/mL PBS for 5 min at 37 °C.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store the Involucrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Antigène
    Involucrin (IVL)
    Autre désignation
    Involucrin (IVL Produits)
    Synonymes
    anticorps INV, anticorps NPH2, anticorps NPHP2, anticorps 1110019C06Rik, anticorps IVL, anticorps Nphp2, anticorps inv, anticorps involucrin, anticorps inversin, anticorps IVL, anticorps INVS, anticorps Ivl, anticorps Invs
    Sujet
    This antibody recognizes a protein of 66-170  kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120  kDa protein. It stains the involucrin in a variety of sizes: 170  kDa in MCF-7 cells, a doublet of ~115 and 150  kDa in gorilla and owl monkey, 66  kDa in dog, and a doublet of 105  kDa in pig. Its epitope maps between amino acids 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
    ID gène
    3713
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