MYL9 anticorps (AA 12-27)

N° du produit ABIN303105

Détail du produit anti-MYL9 anticorps

Antigène: MYL9 (Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9))

Épitope: AA 12-27

Reactivité: Humain, Souris, Rat, Avian, Mammifères

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MYL9 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificité: Amino acids 12-27 of Human Myosin light chain protein.

Réactivité croisée (Details): Species reactivity (expected):Avian, Mammal, Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic peptide - KLH conjugated.

Information d'application

Indications d'application: ELISA (1/5000-1/20000). Western Blot (1/500-1/2000). Immunoprecipitation. Immunohistochemistry on Paraffin Sections (2.5 μg/mL). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by Alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.71 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preserevative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur MYL9

Antigène: MYL9 (Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9))

Autre désignation: MYL9 (MYL9 Produits)

Synonymes: anticorps LC20, anticorps MLC-2C, anticorps MLC2, anticorps MRLC1, anticorps MYRL2, anticorps AI327049, anticorps MLC20, anticorps Mylc2c, anticorps RLC-C, anticorps myosin, anticorps regulatory, anticorps MRCL3, anticorps MRLC2, anticorps MYL9, anticorps zgc:103467, anticorps myl9, anticorps zgc:77916, anticorps myosin light chain 9, anticorps myosin, light polypeptide 9, regulatory, anticorps myosin light chain 9 L homeolog, anticorps myosin, light chain 9, regulatory, anticorps myosin, light chain 12A, regulatory, non-sarcomeric, anticorps myosin, light chain 9a, regulatory, anticorps myosin, light chain 9b, regulatory, anticorps MYL9, anticorps Myl9, anticorps myl9.L, anticorps MYL12A, anticorps myl9a, anticorps myl9b

Sujet: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/ cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, Mouse and Rat have almost identical sequences.Synonyms: LC20, MLC-2C, MLC2, MRLC1, MYRL2, Myosin RLC, Myosin regulatory light chain 2, Myosin regulatory light chain MRLC1, Myosin regulatory light polypeptide 9, smooth muscle

ID gène: 10398

NCBI Accession: NP_006088

UniProt: P24844