ERK1 anticorps (Middle Region)

N° du produit ABIN3044377

Détail du produit anti-ERK1 anticorps

Antigène: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Épitope: AA 287-301, Middle Region

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Fonction: Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase 3(MAPK3) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Séquence: KTKVAWAKLF PKSDS

Réactivité croisée (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase 3(MAPK3) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: mitogen-activated protein kinase 3
Protein Name: Mitogen-activated protein kinase 3(MAP kinase 3/MAPK 3)

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human ERK1(287-301aa KTKVAWAKLFPKSDS), identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Références pour anticorps anti-ERK1 (ABIN3044377)

Han, Wu, Chang, Zhu, Xiao, Li, Zhang, Zhang: "Disulfiram inhibits TGF-β-induced epithelial-mesenchymal transition and stem-like features in breast cancer via ERK/NF-κB/Snail pathway." dans: Oncotarget, Vol. 6, Issue 38, pp. 40907-19, (2016) (PubMed).

Tang, Zhang, Qi, Chen, Li, Liu, He, Li, Zu: "Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line." dans: Experimental and therapeutic medicine, Vol. 9, Issue 5, pp. 1851-1856, (2015) (PubMed).

Liu, Zhu, Xiong, Zheng, Hu, Qiu: "Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells." dans: International journal of clinical and experimental pathology, Vol. 7, Issue 9, pp. 5750-61, (2014) (PubMed).

Ma, Wang, Chen, Chen, Wang, Aisa: "Icariin and icaritin stimulate the proliferation of SKBr3 cells through the GPER1-mediated modulation of the EGFR-MAPK signaling pathway." dans: International journal of molecular medicine, Vol. 33, Issue 6, pp. 1627-34, (2014) (PubMed).

Yang, Han, Liu, Hu, Li, Zhu: "Negative pressure wound therapy is associated with up-regulation of bFGF and ERK1/2 in human diabetic foot wounds." dans: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, Vol. 22, Issue 4, pp. 548-54, (2014) (PubMed).

Li, Zhu, Liu, Liu, Wang, Xiong, Shen, Hu, Zheng: "ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299." dans: International journal of clinical and experimental pathology, Vol. 6, Issue 11, pp. 2460-7, (2013) (PubMed).

Hu, Feng, Tang, Zhu, Lin, Yu: "LIF upregulates expression of NK-1R in NHBE cells." dans: Mediators of inflammation, Vol. 2006, Issue 5, pp. 84829, (2007) (PubMed).

Détails sur ERK1

Antigène: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Autre désignation: MAPK3 (MAPK3 Produits)

Synonymes: anticorps ERK-1, anticorps ERK1, anticorps ERT2, anticorps HS44KDAP, anticorps HUMKER1A, anticorps P44ERK1, anticorps P44MAPK, anticorps PRKM3, anticorps p44-ERK1, anticorps p44-MAPK, anticorps Erk-1, anticorps Erk1, anticorps Ert2, anticorps Esrk1, anticorps Mnk1, anticorps Mtap2k, anticorps Prkm3, anticorps p44, anticorps p44erk1, anticorps p44mapk, anticorps ERK3, anticorps ERK6, anticorps P38GAMMA, anticorps PRKM12, anticorps SAPK-3, anticorps SAPK3, anticorps fi06b09, anticorps wu:fi06b09, anticorps zERK1, anticorps Tb08.10J17.940, anticorps MAPK1, anticorps MNK1, anticorps AW123708, anticorps Erk6, anticorps P38gamma, anticorps Prkm12, anticorps Sapk3, anticorps ATMAPK3, anticorps ATMPK3, anticorps T6D9.4, anticorps mitogen-activated protein kinase 3, anticorps mitogen-activated protein kinase 3, anticorps mitogen-activated protein kinase 12, anticorps mitogen activated protein kinase 3, anticorps mitogen-activated serine/threonine-protein kinase, anticorps MAPK3, anticorps Mapk3, anticorps MAPK12, anticorps mapk3, anticorps Tc00.1047053509475.10, anticorps Tb927.8.3550, anticorps Mapk12, anticorps CEK1, anticorps MPK3

Sujet: Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene.The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases(ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. MAPK3 gene is mapped to human chromosome 16 by hybrid cell panel analysis.

Synonyms: ERK 1 antibody|ERK antibody|ERK-1 antibody|ERK1 antibody|ERT 2 antibody|ERT2 antibody|Extracellular Signal Regulated Kinase 1 antibody|Extracellular signal related kinase 1 antibody|Extracellular signal-regulated kinase 1 antibody|HGNC6877 antibody|HS44KDAP antibody|HUMKER1A antibody|Insulin Stimulated MAP2 Kinase antibody|Insulin-stimulated MAP2 kinase antibody|MAP kinase 1 antibody|MAP kinase 3 antibody|MAP Kinase antibody|MAP kinase isoform p44 antibody|MAPK 1 antibody|MAPK 3 antibody|MAPK antibody|MAPK1 antibody|Mapk3 antibody|MGC20180 antibody|Microtubule Associated Protein 2 Kinase antibody|Microtubule-associated protein 2 kinase antibody|Mitogen Activated Protein Kinase 3 antibody|Mitogen-activated protein kinase 1 antibody|Mitogen-activated protein kinase 3 antibody|MK03_HUMAN antibody|OTTHUMP00000174538 antibody|OTTHUMP00000174541 antibody|p44 ERK1 antibody|p44 MAPK antibody|p44-ERK1 antibody|p44-MAPK antibody|P44ERK1 antibody|P44MAPK antibody|PRKM 3 antibody|PRKM3 antibody|Protein Kinase Mitogen Activated 3 antibody

UniProt: P27361

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins