Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Occludin anticorps (C-Term)

OCLN Reactivité: Humain, Souris, Rat, Chien WB, ELISA, IHC, IP, IF, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN305888
  • Antigène Voir toutes Occludin (OCLN) Anticorps
    Occludin (OCLN)
    Épitope
    • 16
    • 16
    • 14
    • 9
    • 7
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 104
    • 65
    • 45
    • 34
    • 18
    • 12
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    Humain, Souris, Rat, Chien
    Hôte
    • 86
    • 20
    • 3
    • 1
    Lapin
    Clonalité
    • 84
    • 26
    Polyclonal
    Conjugué
    • 46
    • 10
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp Occludin est non-conjugé
    Application
    • 88
    • 33
    • 29
    • 26
    • 26
    • 16
    • 14
    • 14
    • 11
    • 8
    • 7
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.
    Purification
    Immunoaffinity purified
    Immunogène
    A GST fusion protein consisting of the C-terminal 150 amino acids of human occludin fused to GST.

    Type of Immunogen: Fusion protein
    Isotype
    IgG
    Top Product
    Discover our top product OCLN Anticorps primaire
  • Indications d'application
    Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-P (2.5 μg/mL), IP, WB (1 - 3 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.
    Commentaires

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS,  pH 7.4, 0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeat freeze-thaw cycles.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Antigène
    Occludin (OCLN)
    Autre désignation
    OCLN / Occludin (OCLN Produits)
    Synonymes
    anticorps AI503564, anticorps Ocl, anticorps ocln, anticorps oclnb, anticorps tpmt, anticorps OCLN, anticorps wu:fd23h10, anticorps wu:fi13c01, anticorps zgc:113992, anticorps zgc:56359, anticorps BLCPMG, anticorps occludin, anticorps occludin S homeolog, anticorps occludin a, anticorps Ocln, anticorps OCLN, anticorps ocln.S, anticorps ocln, anticorps oclna
    Sujet
    Name/Gene ID: OCLN

    Synonyms: OCLN, Occludin, BLCPMG
    ID gène
    100506658
    UniProt
    Q16625
    Pathways
    Cell-Cell Junction Organization, Hepatitis C
Vous êtes ici:
Support technique