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KRT8 anticorps

KRT8 Reactivité: Humain, Souris WB, IHC, FACS, ICC, IHC (fro) Hôte: Souris Monoclonal M20 unconjugated
N° du produit ABIN335327
  • Antigène Voir toutes KRT8 Anticorps
    KRT8 (Keratin 8 (KRT8))
    Reactivité
    • 247
    • 81
    • 75
    • 32
    • 32
    • 30
    • 30
    • 16
    • 14
    • 10
    • 7
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain, Souris
    Hôte
    • 132
    • 124
    • 2
    • 1
    Souris
    Clonalité
    • 129
    • 128
    Monoclonal
    Conjugué
    • 137
    • 23
    • 17
    • 9
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Cet anticorp KRT8 est non-conjugé
    Application
    • 163
    • 97
    • 88
    • 82
    • 67
    • 50
    • 41
    • 40
    • 31
    • 29
    • 22
    • 18
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificité
    Human and mouse.
    Purification
    Purified
    Immunogène
    M20 is a mouse monoclonal IgG1 antibody derived by fusion of murine myeloma cells with spleen cells from a mouse immunized with keratin isolated from the human breast carcinoma cell line MCF-7.
    Clone
    M20
    Isotype
    IgG1
  • Indications d'application
    M20 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells. M20 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
    Restrictions
    For Research Use only
  • Stock
    4 °C
  • Waseem, Karsten, Leigh, Purkis, Waseem, Lane: "Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability." dans: Biochemistry, Vol. 43, Issue 5, pp. 1283-95, (2004) (PubMed).

    Ku, Gish, Wright, Omary: "Keratin 8 mutations in patients with cryptogenic liver disease." dans: The New England journal of medicine, Vol. 344, Issue 21, pp. 1580-7, (2001) (PubMed).

    Wetzels, Kuijpers, Lane, Leigh, Troyanovsky, Holland, van Haelst, Ramaekers: "Basal cell-specific and hyperproliferation-related keratins in human breast cancer." dans: The American journal of pathology, Vol. 138, Issue 3, pp. 751-63, (1991) (PubMed).

    Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." dans: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, (1990) (PubMed).

    Schaafsma, Ramaekers, van Muijen, Lane, Leigh, Robben, Huijsmans, Ooms, Ruiter: "Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression." dans: The American journal of pathology, Vol. 136, Issue 2, pp. 329-43, (1990) (PubMed).

    Smedts, Ramaekers, Robben, Pruszczynski, van Muijen, Lane, Leigh, Vooijs: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." dans: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, (1990) (PubMed).

    Ivanyi, Groeneveld, Van Doornewaard, Mooi, Hageman: "Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis." dans: Cancer research, Vol. 50, Issue 16, pp. 5143-52, (1990) (PubMed).

    Schaafsma, Ramaekers, van Muijen, Ooms, Ruiter: "Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract." dans: Histochemistry, Vol. 91, Issue 2, pp. 151-9, (1989) (PubMed).

    Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, (1987) (PubMed).

    Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." dans: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, (1987) (PubMed).

  • Antigène
    KRT8 (Keratin 8 (KRT8))
    Autre désignation
    Cytokeratin 8 / Keratin K8 (KRT8 Produits)
    Synonymes
    anticorps CARD2, anticorps CK-8, anticorps CK8, anticorps CYK8, anticorps K2C8, anticorps K8, anticorps KO, anticorps AA960620, anticorps AL022697, anticorps AU019895, anticorps Card2, anticorps EndoA, anticorps Krt-2.8, anticorps Krt2-8, anticorps CYKER, anticorps KRT2-8, anticorps KERATIN8, anticorps ck8, anticorps cyk8, anticorps k2c8, anticorps card2, anticorps krt2-5, anticorps MGC69490, anticorps KRT8, anticorps DreK8, anticorps cb186, anticorps krt2-8, anticorps sb:cb186, anticorps wu:fa20h05, anticorps wu:fa95h10, anticorps wu:fb96c06, anticorps zf-K8, anticorps zfk8, anticorps DKFZp468F2127, anticorps keratin 8, anticorps keratin 8 S homeolog, anticorps KRT8, anticorps Krt8, anticorps krt8.S, anticorps krt8
    Sujet
    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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