PARK7/DJ1 anticorps

N° du produit ABIN335354

Détail du produit anti-PARK7/DJ1 anticorps

Antigène: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Reactivité: Humain, Souris, Chien, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PARK7/DJ1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Human, mouse, dog and Xenopus laevis.

Purification: Serum

Immunogène: 12B11F8 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to amino acid residues 779-793 of human plakophilin-3 coupled to keyhole limpet hemocyanin. (KLHRDFRAKGYRKED).

Clone: 12B11F8

Isotype: IgG1

Information d'application

Indications d'application: 12B11F8 reacts with an epitope located between residues 779-793 in human plakophilin-3. 12B11F8 is suitable for immunocytochemistry (methanol-fixed cells), immunohistochemistry on frozen tissues, immunoprecipitation and Western blot detection. For optimal results, fixed cells or tissues should be treated with 0.2% Triton-X 100 for 5 min. prior to incubation with primary antibody. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-PARK7/DJ1 (ABIN335354)

Bonné, Gilbert, Hatzfeld, Chen, Green, van Roy: "Defining desmosomal plakophilin-3 interactions." dans: The Journal of cell biology, Vol. 161, Issue 2, pp. 403-16, (2003) (PubMed).

Bonné, van Hengel, Nollet, Kools, van Roy: "Plakophilin-3, a novel armadillo-like protein present in nuclei and desmosomes of epithelial cells." dans: Journal of cell science, Vol. 112 ( Pt 14), pp. 2265-76, (1999) (PubMed).

Détails sur PARK7/DJ1

Antigène: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Autre désignation: PARK7 / DJ-1 (PARK7 Produits)

Synonymes: anticorps DJ-1, anticorps DJ1, anticorps CAP1, anticorps Dj1, anticorps SP22, anticorps dj1, anticorps zgc:103725, anticorps park7b, anticorps park7, anticorps park7a, anticorps Parkinsonism associated deglycase, anticorps Parkinson disease (autosomal recessive, early onset) 7, anticorps parkinson protein 7, anticorps parkinson protein 7 S homeolog, anticorps parkinson protein 7 L homeolog, anticorps PARK7, anticorps Park7, anticorps park7, anticorps park7.S, anticorps park7.L

Sujet: Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells [1]. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Proton Transport